Anti-MEK2 (Phospho-Thr394) Antibody (43008)

Anti-MEK2 (Phospho-Thr394) Antibody (43008)

Product No.: 43008

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Target
MEK2 (Phospho-Thr394)
Product Type
Polyclonal
Isotype
Rabbit
Applications
WB,IHC

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Select Product Size

Data

Immunoblot: Detection of MEK2 (phospho-Thr394) in HepG2 and HeLa cell extracts untreated or treated with UV.
Immunohistochemistry: Detection of MEK2 (phospho-Thr394) in paraffin-embedded human breast carcinoma tissue.
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Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
Peptide sequence that includes phosphorylation site of threonine (P-G-T(p)-P-T) derived from human MEK2 and conjugated to KLH.
Product Concentration
1.0 mg/ml
Formulation
PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
State of Matter
Liquid
Product Preparation
Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Storage and Handling
This antibody is stable for at least one (1) year at -20°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Western blotting: use at dilution of 1:500- 1:1,000. A band of ~44kDa is detected.

Immunohistochemistry: use at dilution of 1:50- 1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to MEK2 (Phospho-Thr394)
Background
MEK2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase plays an important role in mitogen growth factor signal transduction. It phosphorylates and activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax.

Antigen Details

Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126). {UniProtKB:Q63932, PubMed:29433126}.
NCBI Gene Bank ID
UniProt.org
Research Area
Phosphospecific Antibodies

References & Citations

WB,IHC
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.