Anti-Mouse Angiopoietin-3
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen E. coli-Derived Recombinant Mouse Angiopoietin-3 Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828137 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse ANG-3 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse ANG-3 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Mouse Angiopoietin-3 (ANG-3) recognizes Mouse ANG-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Angiopoietins 3 (Ang-3) is a secretory protein1 and a ligands of Tie-2 receptor tyrosine kinase.2 Ang3 is most abundant in adrenal gland, placenta, thyroid gland, heart and small intestine.1 It inhibits endothelial cell proliferation and survival and blocks Ang-1.2 PubMed NCBI Gene Bank ID References & Citations1. Koh, GY. et al. (1999) FEBS Lett. 443: 353 2. Yu, Q. et al. (2004) Cancer Res. 64: 6119 Technical ProtocolsIHC FFPE  Certificate of Analysis |
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