Anti-Mouse CCL21 – (Clone 59106)

Pricing & Details

Product No.C1421
Clone
59106
Protein
CCL21
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
6Ckine, CKb9, ECL, MGC34555, SCYA21, SLC, TCA4, Exodus-2, Chemokine (C-C Motif) Ligand 21
Isotype
Rat
IgG2b
Applications
CyTOF®
,
ELISA Cap
,
FC
,
IHC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1421-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
C1421-100 µg
100 µg
$430.00
In stock
Max:
Min: 1
Step: 1
C1421-500 µg
500 µg
$1,025.00
In stock
Max:
Min: 1
Step: 1
Contact Us For Academic mAb Advantage Pricing

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse CCL21 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:C1500 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:E114.
Western Blotting: To detect Mouse CCL21 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CCL21 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E114.
Flow Cytometry: Suitable for use at concentration of 2.5 µg/106 cells.
Other Applications Reported In Literature ?
Immunohistochemistry:Suitable for use at concentration of 25 µg/mL.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Chemokine (C-C Motif) Ligand 21 (CCL21) (Clone 59106) recognizes an epitope on Mouse CCL21. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). CCL21 elicits its effects by binding to a cell surface chemokine receptor known as CCR7.

In the cancer biology CCL21 seems to have multifaceted roles. CCL21 attracts CCR7 bearing cells especially T and dendritic cells but also various cancer cells. Besides the antitumour role as leukocyte recruiting, CCL21 has been shown to facilitate dendritic cell functions and to exert an angiostatic effect.

Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity.

Antigen Details

Protein
PubMed
Elisa Sandwich Protocol
Flow Cytometry
IHC
General Western Blot Protocol

Formats Available

Prod No.
Description
C1421
Products are for research use only. Not for use in diagnostic or therapeutic procedures.