Anti-Mouse CD11a (Clone I21/7) – Purified in vivo PLATINUM™ Functional Grade

Clone I21/7 is an antibody that recognizes mouse CD11a, the α subunit of LFA-1, a crucial adhesion molecule involved in immune cell interactions. Common in vivo applications of Clone I21/7 in mice include:

• Immune Cell Depletion: The antibody is used to deplete or block CD11a-expressing cells in vivo, which helps in studying immune responses and interactions.
• Functional Studies: It is applied in functional assays to understand the role of LFA-1 in immune cell adhesion and signaling, particularly in processes like immunological synapses between T cells and antigen-presenting cells.
• Therapeutic Interventions: Clone I21/7 can be utilized to evaluate therapeutic interventions targeting CD11a-expressing immune cell populations, providing insights into disease mechanisms and potential treatments.

These applications are pivotal for immunologists and stem cell researchers conducting mouse studies aimed at dissecting complex biological processes and developing new therapies.

Commonly used antibodies or proteins paired with I21/7 (anti-mouse CD11a, LFA-1α) in the literature include antibodies targeting other leukocyte adhesion molecules, T cell and activation markers, and cytokines relevant for analyzing immune cell function and trafficking.

Relevant context:

• I21/7 is a monoclonal antibody against mouse CD11a (LFA-1α), a key adhesion molecule involved in immune cell trafficking and T cell activation.

Commonly used companion antibodies/proteins:

• Anti-CD18 (LFA-1β): Often paired with anti-CD11a to study the LFA-1 integrin complex, since CD11a and CD18 form a functional heterodimer.
• Anti-CD3: Used to identify or stimulate T cells during lymphocyte activation or trafficking studies (CD3 is a T cell co-receptor).
• Anti-CD8 and Anti-CD4: For subset analysis of cytotoxic and helper T lymphocytes, commonly co-stained with CD11a to investigate expression on different T cell populations.
• Anti-CD45: Used to broadly distinguish leukocytes; co-labeling with CD11a reveals adhesion dynamics across leukocyte types.
• Cytokine-related reagents: In studies investigating immune modulation or trafficking, antibodies or recombinant proteins for cytokines like IL-2, IL-21, IL-7, and IL-15 may be employed to dissect T cell responses.

Experimental pairings are typically designed to:

• Map immune cell populations (using lineage and activation markers).
• Analyze cell adhesion, extravasation, or tissue infiltration (using additional integrin or chemokine receptor antibodies).
• Study functional responses (using cytokine detection/neutralization antibodies).

These combinations reflect broad immunological practice and are consistently reported in primary immunology literature and antibody product datasheets. For precise applications (e.g., blockade of cell trafficking during inflammation or cancer studies), co-administration of antibodies against VLA-4 (CD49d), ICAM-1 (CD54), or chemokine receptors (e.g., CCR7, CXCR3) are also common.

If you seek details for a particular research context (e.g., flow cytometry, in vivo blockade, cancer models), the panel may be further specialized.

Key Findings from Clone I21/7 Citations in Scientific Literature

Based on the available search results, there is no direct reporting of key scientific findings from clone I21/7 (anti-mouse CD11a/LFA-1α antibody) in peer-reviewed literature within the provided sources. The main information available pertains to the technical and biological characteristics of the antibody, not its use in producing novel scientific discoveries.

Here is a summary of the available details:

• Specificity and Functional Role: Clone I21/7 specifically recognizes mouse CD11a (LFA-1α), a critical component of the LFA-1 integrin complex, which is essential for immune cell adhesion, migration, and activation. LFA-1 is involved in immunological synapse formation between CD8+ T cells and antigen-presenting cells, natural killer cell cytotoxicity, leukocyte-endothelial cell interactions, and cytotoxic T-cell-mediated killing.
• Technical Use: The antibody is used in research to study immune cell interactions and functions mediated by CD11a. Titration is recommended for optimal performance, and the optimal working dilution may vary by application.
• No Reported Novel Discoveries: None of the search results describe key experimental findings or novel biological insights generated specifically through the use of clone I21/7. The main scientific literature does not appear to highlight breakthrough results attributed to this clone.

Conclusion

Clone I21/7 is a well-characterized tool for studying the role of CD11a in immune cell biology, but there are no prominent scientific findings directly credited to this clone in the provided sources. Its value lies in its specificity for mouse CD11a, enabling researchers to dissect the contributions of LFA-1 to immune responses in experimental models. For detailed experimental outcomes, primary literature using this clone would need to be examined directly, as general vendors’ descriptions do not report such findings.

The search results do not specifically discuss the dosing regimens of clone I21/7 across different mouse models. However, clone I21/7 is an antibody targeting Mouse CD11a, also known as LFA-1α, and is used in various immunological studies in mice. When considering dosing regimens generally for antibodies and cytokines in mouse models, doses and regimens can vary widely depending on the specific application and model.

For IL-21, a cytokine used in cancer immunotherapy, dosing regimens can include daily or three times weekly injections with doses ranging from 12 to 50 µg per dose, depending on the model and the desired therapeutic effect. For antibodies, such as those used in checkpoint blockade therapies, typical doses range from 100 to 500 µg per mouse, with injections often administered every 3-4 days intraperitoneally.

Since specific information about clone I21/7 dosing regimens in different mouse models isn't provided, it's essential to consult the literature or specific studies using this clone to determine the most appropriate dosing regimen for a particular experimental design.