Anti-Mouse CD11a (Clone I21/7) – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse CD11a (Clone I21/7) – Purified in vivo PLATINUM™ Functional Grade

Product No.: C976

[product_table name="All Top" skus="C376"]

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Clone
I21/7
Target
CD11a
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
LFA-1α
Isotype
Rat IgG2a
Applications
FA
,
FC
,
in vivo

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Recommended Isotype Controls
Recommended Dilution Buffer
Immunogen
Whole T cells Lymphoma BW5147 cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for clone I21/7 antibody for staining cells in flow cytometry is ≤ 0.5-1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
IP The antibody precipitates a (Mr 150/90 kDa) heterodimer from mouse leukocytes.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone I21/7 recognizes an epitope on mouse CD11a.
Background
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
Antigen Distribution
CD11a is present on thymocytes, blood lymphocytes, bone marrow cells and certain lymphoma and macrophage-like cell lines.
Ligand/Receptor
ICAM-1 (CD54), ICAM-2(CD102), ICAM-3(CD50)
Function
Intercellular adhesion
PubMed
NCBI Gene Bank ID
Research Area
Cell Adhesion
.
Cell Biology
.
Costimulatory Molecules
.
Immunology
.
Signal Transduction

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

Clone I21/7 is a monoclonal antibody used in in vivo mouse studies to target CD11a, which is the ?-subunit of LFA-1, an integrin involved in lymphocyte adhesion, migration, and immunological synapse formation.

Essential details about its use:

  • Specificity: Clone I21/7 recognizes mouse CD11a (LFA-1?), a molecule widely expressed on thymocytes, blood lymphocytes, bone marrow cells, and macrophage-like cell lines.
  • Functional Role in Studies:
    • It is mainly utilized to block or modulate LFA-1-mediated adhesion reactions (interaction with ICAM-1 and ICAM-2), allowing researchers to interrogate processes such as immune cell trafficking, T cell activation, and effector functions in vivo.
    • By inhibiting CD11a, studies can assess T cell migration, neutrophil recruitment, and general leukocyte-endothelial interactions during immune responses.
  • Applications:
    • Designed specifically for in vivo functional studies: the antibody is produced with very low endotoxin content and high purity to minimize off-target effects and ensure reliable results in live animal experiments.
    • Used in protocols like IP (immunoprecipitation), WB (Western blot), B (blocking), FA (functional assays), although the blocking and functional applications are most relevant for in vivo work.
  • Technical Notes:
    • Clone I21/7 is a rat IgG2a isotype, which is useful for avoiding cross-reactivity and for compatibility with mouse model immunology.
    • High purity and endotoxin removal are emphasized to support safe and reproducible in vivo use.

Typical In Vivo Experimental Uses:

  • Administration to mice (most often C57BL/6 strains) by injection to block CD11a function, exploring consequences for immune cell trafficking, inflammation, or host defense during conditions such as infection, autoimmunity, and graft-versus-host disease.
  • Facilitation of mechanistic studies of lymphocyte interactions by disrupting immunological synapses and leukocyte adhesion, helping unravel molecular control of immune responses.

No specific published mouse study with Clone I21/7 was found in the provided results, but standard product descriptions and technical applications indicate the antibody is primarily used to probe the functional role of CD11a/LFA-1 in live mice and is suitable for antibody-mediated blocking experiments.

The correct storage temperature for a sterile packaged clone such as I21/7 is between 2°C and 8°C (36°F to 46°F), which is the standard range for refrigerated storage of sterile products.

This range is widely recognized in pharmaceutical and laboratory practices for maintaining the sterility and viability of biological materials, including plant and tissue clones, unless product labeling specifies otherwise. If you are storing plant tissue or cuttings for longer-term viability, storage in a standard refrigerator (within this temperature range) is also a common horticultural practice.

  • Refrigerator (cold storage): 2°C to 8°C (36°F to 46°F)
  • Always check the specific product labeling for exceptions or special instructions.

Storing outside this range (such as in a freezer or at room temperature) may compromise the sterility or viability of the clone unless explicitly stated by the manufacturer or supplier.

Commonly used antibodies or proteins co-studied with I21/7 in the literature—assuming you refer to the cytokines IL-21 and IL-7 (as I21/7 is not a standard antibody/protein designation and is interpreted here as a pairing of these gamma-chain family cytokines)—include other cytokines from the same family, especially IL-15 and IL-2.

Key proteins and antibodies frequently combined or compared with IL-21 and IL-7:

  • IL-15: Frequently combined with either IL-7 or IL-21 in trifunctional antibody-cytokine fusion protein studies, particularly in cancer immunotherapy settings. These combinations are designed to enhance cooperative activation and proliferation of T cells and cytotoxic responses.
  • IL-2: Used in parallel or comparison with IL-21 and IL-15 since these cytokines share common receptor subunits and similar immunostimulatory functions. IL-2, IL-15, and IL-21 are often benchmarked in studies examining their effects on T cell activation and anti-tumor responses.
  • Trifunctional antibody fusion proteins: These often include one or two cytokines (such as IL-15/IL-21 or IL-15/IL-7) attached to single-chain variable fragment (scFv) antibodies for targeted delivery in cancer models.
  • F8 antibody: Used in combination with IL-21 for tumor targeting by recognizing the EDA domain of fibronectin, showing that the format and pairing significantly impact therapeutic efficacy.

Other relevant proteins:

  • TRIM21: While not directly paired with IL-21 or IL-7, TRIM21 is an intracellular antibody receptor that can be involved in studies detailing mechanisms of antibody-dependent cellular immunity, sometimes in the context of cytokine-mediated immune responses.

These studies emphasize the importance of combinatorial approaches using gamma-chain cytokines (IL-2, IL-7, IL-15, IL-21) with tumor-targeting antibodies to boost specific leukocyte subsets' function, particularly in cancer immunotherapy.

If you were referring to a different "I21/7" antibody (such as a clone designation or proprietary antibody), please clarify for more specific details. The current answer focuses on commonly co-used proteins with IL-21 and IL-7 based on prevailing literature usage.

The scientific literature referencing clone I21/7 is limited in the available search results, and there is no direct discussion specifically of "clone I21/7" in the sources provided. However, based on broader context from the search results covering molecular cloning of HIV-1 and the importance of reference clones, the following key findings can be synthesized:

  • Reference Clones in HIV-1 Research: Reference HIV-1 clones—such as those discussed in the cited literature—are critical for studies involving non-subtype B viruses. Such molecular clones enable standardized phylogenetic, immunological, and biological analyses, which are essential for understanding virus diversity and for vaccine development.
  • Scarcity of Non-Subtype B Clones: There has historically been a limited number of available full-length, nonrecombinant molecular clones for non-subtype B HIV-1 subtypes. The field has recognized this gap, and efforts have focused on expanding the repertoire of subtype-specific molecular reagents, as reflected in the cloning and analysis of panels of near-full-length HIV-1 genomes from diverse global isolates.
  • Characterization and Utility: Detailed phylogenetic analyses of these clones have shown both nonmosaic representatives of major subtypes and the prevalence of complex intersubtype recombinants. This highlights the extensive genetic diversity of circulating HIV-1 strains—an important consideration for vaccine design and immunological research.
  • Research on Biological and Functional Relevance: The ability to generate replication-competent proviruses from such clones is crucial for biological and functional studies, particularly when evaluating correlates of immune protection and in vitro replication kinetics.

Note: No search results specifically mention "clone I21/7" by name, nor do they directly provide citations analyzing its specific scientific impact. The above findings are based on broader contextual information about the role and importance of reference HIV-1 clones discussed in the provided literature. If "clone I21/7" was intended to refer to a specific HIV-1 molecular clone, its direct citation and key findings do not appear in these sources.

For a more comprehensive analysis, direct identification of publications that cite or analyze "clone I21/7" would be required, which may involve more targeted literature databases or clarification on the clone's identity.

References & Citations

1. Trowbridge, I.S. et al. (1981) J. Exp. Med. 154:1517 2. Springer, T. et al. (1982) Immunol. Rev. 68: 171 3. Wuthrich, R. P. et al. (1991) Cell Immunol. 144:22 3. Falanga, P. B. et al. (1991) Eur. J. Immunol. 21: 2259 4. Talento, A. et al. (1993) Transplantation. 55: 418 5. Barret, T.B. et al. (1991) J. Immunol. 146: 1722
FA
Flow Cytometry
in vivo Protocol

Certificate of Analysis

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Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.