Anti-Mouse CD11a (Clone I21/7) – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD11a (Clone I21/7) – Purified in vivo PLATINUM™ Functional Grade
Product No.: C976
Clone I21/7 Target CD11a Formats AvailableView All Product Type Monoclonal Antibody Alternate Names LFA-1α Isotype Rat IgG2a Applications FA , FC , in vivo |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Whole T cells Lymphoma BW5147 cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829886 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for clone I21/7 antibody for staining cells in flow cytometry is ≤ 0.5-1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IP The antibody precipitates a (Mr 150/90 kDa) heterodimer from mouse leukocytes. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone I21/7 recognizes an epitope on mouse CD11a. Background LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis. Antigen Distribution CD11a is present on thymocytes, blood lymphocytes, bone marrow cells and certain lymphoma and macrophage-like cell lines. Ligand/Receptor ICAM-1 (CD54), ICAM-2(CD102), ICAM-3(CD50) Function Intercellular adhesion PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Costimulatory Molecules . Immunology . Signal Transduction Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone I21/7 is a monoclonal antibody used in in vivo mouse studies to target CD11a, which is the ?-subunit of LFA-1, an integrin involved in lymphocyte adhesion, migration, and immunological synapse formation. Essential details about its use:
Typical In Vivo Experimental Uses:
No specific published mouse study with Clone I21/7 was found in the provided results, but standard product descriptions and technical applications indicate the antibody is primarily used to probe the functional role of CD11a/LFA-1 in live mice and is suitable for antibody-mediated blocking experiments. The correct storage temperature for a sterile packaged clone such as I21/7 is between 2°C and 8°C (36°F to 46°F), which is the standard range for refrigerated storage of sterile products. This range is widely recognized in pharmaceutical and laboratory practices for maintaining the sterility and viability of biological materials, including plant and tissue clones, unless product labeling specifies otherwise. If you are storing plant tissue or cuttings for longer-term viability, storage in a standard refrigerator (within this temperature range) is also a common horticultural practice.
Storing outside this range (such as in a freezer or at room temperature) may compromise the sterility or viability of the clone unless explicitly stated by the manufacturer or supplier. Commonly used antibodies or proteins co-studied with I21/7 in the literatureassuming you refer to the cytokines IL-21 and IL-7 (as I21/7 is not a standard antibody/protein designation and is interpreted here as a pairing of these gamma-chain family cytokines)include other cytokines from the same family, especially IL-15 and IL-2. Key proteins and antibodies frequently combined or compared with IL-21 and IL-7:
Other relevant proteins:
These studies emphasize the importance of combinatorial approaches using gamma-chain cytokines (IL-2, IL-7, IL-15, IL-21) with tumor-targeting antibodies to boost specific leukocyte subsets' function, particularly in cancer immunotherapy. If you were referring to a different "I21/7" antibody (such as a clone designation or proprietary antibody), please clarify for more specific details. The current answer focuses on commonly co-used proteins with IL-21 and IL-7 based on prevailing literature usage. The scientific literature referencing clone I21/7 is limited in the available search results, and there is no direct discussion specifically of "clone I21/7" in the sources provided. However, based on broader context from the search results covering molecular cloning of HIV-1 and the importance of reference clones, the following key findings can be synthesized:
Note: No search results specifically mention "clone I21/7" by name, nor do they directly provide citations analyzing its specific scientific impact. The above findings are based on broader contextual information about the role and importance of reference HIV-1 clones discussed in the provided literature. If "clone I21/7" was intended to refer to a specific HIV-1 molecular clone, its direct citation and key findings do not appear in these sources. For a more comprehensive analysis, direct identification of publications that cite or analyze "clone I21/7" would be required, which may involve more targeted literature databases or clarification on the clone's identity. References & Citations1. Trowbridge, I.S. et al. (1981) J. Exp. Med. 154:1517
2. Springer, T. et al. (1982) Immunol. Rev. 68: 171
3. Wuthrich, R. P. et al. (1991) Cell Immunol. 144:22
3. Falanga, P. B. et al. (1991) Eur. J. Immunol. 21: 2259
4. Talento, A. et al. (1993) Transplantation. 55: 418
5. Barret, T.B. et al. (1991) J. Immunol. 146: 1722 Technical ProtocolsCertificate of Analysis |
Related Products
Prod No. | Description |
---|---|
S211 | |
R1364 | |
I-1177 | |
C247 | |
F1175 | |
R1214 | |
S571 |
Formats Available
Prod No. | Description |
---|---|
C1876 | |
C223 | |
C224 | |
C1870 | |
C226 | |
C225 | |
C376 | |
C976 |
