Anti-Mouse CD200 (Clone OX-90) – Purified in vivo PLATINUM™ Functional Grade

Clone OX-90 is a rat monoclonal antibody specific for murine CD200 (OX-2), and its most common in vivo applications in mice are for functional blockade of CD200, modulating immune responses, and as a research tool for studying immune regulation and cell populations expressing CD200.

Common in vivo applications of clone OX-90 in mice include:

• Functional blockade of CD200-CD200R interactions: OX-90 is widely used to block the function of mouse CD200 in vivo, helping to study the inhibitory role of the CD200-CD200R signaling axis in immune regulation, particularly in relation to myeloid cell activity, macrophage activation, and the immune suppressive microenvironment. Blocking CD200 increases myeloid and lymphocyte activity, important for understanding autoimmune disorders, tumor immunity, and graft-versus-host disease.

• Modulation of immune responses in disease models: Researchers utilize OX-90 to investigate how CD200 suppression affects cancer progression, autoimmunity, and other immune disorders, since CD200 has a significant role in limiting inflammatory responses and regulating cytokine induction.

• Cell depletion and phenotyping: Due to its specificity for CD200-expressing cells (including resting/activated B cells, thymocytes, certain T cell subsets, dendritic cells, neurons, and endothelium), OX-90 can be used (alone or with complement or secondary reagents) for selective depletion or tracking of these populations in vivo.

• Flow cytometry and immunohistology in vivo: OX-90 is employed for staining and detection of CD200-expressing cells in tissue samples and circulation, supporting both basic and translational research.

Summary Table: Common In Vivo Applications of OX-90 in Mice

Context and Additional Information:

• CD200 is a cell surface glycoprotein with regulatory effects on the immune system, especially through myeloid cell inhibition by interaction with its receptor (CD200R).
• Administration of anti-CD200 OX-90 in mice can disturb this inhibitory signal, supporting research into mechanisms of immune tolerance, immune evasion by tumors, or tissue-specific immune privilege.

Most suppliers emphasize clone OX-90’s use for in vivo functional studies—distinct from in vitro-only clones—due to its low endotoxin profiles and suitability for injection into animals.

All cited applications are for research use only, not diagnostic or therapeutic use.

The antibody OX-90 is a well-characterized monoclonal antibody used for detecting rat CD90 (Thy-1), a surface glycoprotein involved in cell adhesion and signaling, especially in immunological and neurological studies. In the literature, OX-90 is often used alongside several other antibodies or protein markers, typically as part of multi-color flow cytometry or immunohistochemistry panels to distinguish cell types or characterize cell populations.

Commonly used antibodies or proteins with OX-90 include:

• CD45: A ubiquitous leukocyte marker, used to identify hematopoietic cells.
• CD3, CD4, CD8: T cell subset markers (CD3 for total T cells, CD4 for helper T cells, CD8 for cytotoxic T cells).
• CD11b, CD11c: Myeloid and dendritic cell markers.
• CD34, CD117 (c-Kit): Stem and progenitor cell markers in hematopoiesis.
• Gr-1 (Ly6G/Ly6C), CD19, CD20: Markers for neutrophils, B cells, and mature B lymphocytes.
• Isotype Controls: Used to confirm specificity of staining.

These markers are selected based on the biological context (e.g., immunophenotyping, stem cell research, neurobiology), allowing researchers to delineate cell lineages or functional states within complex tissues.

Supporting details:

• OX-90 (rat CD90, also called Thy-1) is primarily used for rat tissue characterization and often multiplexed with other lineage or activation markers in immunological assays.
• Published applications frequently include flow cytometry panels with the above markers to sort, quantify, or profile immune, stromal, or neuronal populations.

If you have a specific experimental context (such as a particular cell type or disease model), I can provide more tailored combinations commonly cited in that field.

The clone OX-90 refers to a rat monoclonal antibody used to detect mouse CD200/OX2, a cell surface antigen. While specific citations for clone OX-90 might not be listed in the results, the antibody is widely used in research related to CD200 and its role in immune regulation. Here are some key points related to CD200 and its studies:

1. CD200 Function: CD200, also known as OX2, is a membrane glycoprotein belonging to the immunoglobulin superfamily. It plays a crucial role in regulating the immune system, particularly in modulating macrophage and granulocyte activation.

2. Expression and Importance: CD200 is expressed on various cell types, including resting and activated B cells, subsets of T cells, neurons, and follicular dendritic cells. Its interaction with CD200R influences immune responses by inhibiting macrophage activation and reducing mast cell degranulation.

3. Applications of OX-90 Antibody: The OX-90 antibody is commonly used in flow cytometry, immunohistochemistry, and other applications to study CD200 expression and function. It is particularly useful for understanding the role of CD200 in immune regulation and its potential therapeutic applications.

4. Research Context: Studies involving CD200 often focus on its regulatory role in immune responses and its potential as a therapeutic target in diseases where immune dysregulation is a factor. However, specific citations directly referencing clone OX-90 may not provide comprehensive key findings without access to the full literature.

In summary, clone OX-90 is a valuable tool in scientific research related to CD200 and immune regulation, but detailed findings from specific citations would require access to broader scientific literature.

There are no direct search results available on the dosing regimens of clone OX-90 in mouse models. Based on the lack of specific information regarding OX-90 in the provided search results, it is not possible to describe how its dosing regimens may vary across different mouse models.

General Considerations for Monoclonal Antibody Dosing in Mice

While OX-90-specific details are unavailable, dosing regimens for monoclonal antibodies in mouse models typically depend on several factors, including the target, antibody clone, mouse strain, injection route, disease model, and experimental goals. Reference to dosing guides for other monoclonal antibodies (e.g., 9H10, 9D9) shows that standard doses often range from 100 to 250 μg per mouse, administered intraperitoneally (i.p.), with dosing intervals such as every 3 days being common for checkpoint inhibitors like anti-CTLA-4 antibodies. These parameters are adjusted based on the antibody’s mechanism (e.g., depleting or blocking) and the desired pharmacological or immunological effect.

Recommendations for Establishing a Dosing Regimen

• Consult primary literature or manufacturer guidelines: For novel or less common clones like OX-90, always refer to the original research publications or the antibody supplier’s technical datasheets for recommended doses and schedules.
• Pilot experiments: Conduct dose-ranging studies to determine the minimally effective and maximally tolerated doses in your specific model.
• Model-specific adjustments: Consider the disease or biological context (e.g., cancer, immunology, neurology) and the mouse strain when designing the regimen, as these can significantly affect pharmacokinetics and pharmacodynamics.
• Monitoring and refinement: Regularly monitor animal health, target engagement, and therapeutic outcomes to refine the dosing schedule.

Summary Table: Typical Monoclonal Antibody Dosing in Mice

Conclusion

No specific information on OX-90 dosing regimens in mouse models is available in the provided search results. Researchers should rely on primary literature, manufacturer recommendations, and preliminary dosing studies tailored to their specific experimental model and objectives. If OX-90 is a proprietary or less common clone, direct communication with the supplier or consulting recent publications using this clone is advised.