Anti-Mouse CD200 (Clone OX-90) – Purified in vivo PLATINUM™ Functional Grade

The clone OX-90, an anti-mouse CD200 antibody, is used in in vivo mouse studies primarily for its ability to target CD200 (also known as OX-2), an immune suppressor molecule that plays a role in regulating the induction of cytokine production by dendritic cells. It helps limit pro-inflammatory signals and is involved in the regulation of immune responses, which can be crucial in studies related to immune disorders and cancer.

Key Uses of OX-90 in In Vivo Studies:

1. Modulating Immune Responses: By targeting CD200, investigators can study alterations in immune responses, particularly in contexts where CD200's role in inhibiting pro-inflammatory signals is critical.

2. Cancer Research: CD200's involvement in immune suppression can be exploited to study cancer models where immune evasion is a factor. Blocking CD200 with antibodies like OX-90 could enhance anti-tumor immune responses.

3. Neurological Studies: Since CD200 is also expressed on neurons, OX-90 can be used to study neurological conditions where immune responses are altered.

4. Flow Cytometry (FC) and Immunohistochemistry (IHC): OX-90 is applicable for these techniques, allowing researchers to quantify CD200 expression and localize it in tissues, respectively.

Preparation and Handling:

• Concentration: The antibody is typically provided at concentrations ranging from 1.0 to 5.0 mg/ml, with ultra-low endotoxin versions available at ? 2.0 mg/ml.
• Formulation: Supplied in a sterile, preservative-free solution of PBS, making it suitable for in vivo administration.
• Storage: Short-term storage at 2-8°C; long-term storage at –80°C is recommended to avoid repeated freeze-thaw cycles.

The correct storage temperature for the sterile packaged clone OX-90, an anti-mouse CD200 antibody, is between 2°C and 8°C. It should be protected from prolonged exposure to light and not frozen. This ensures the stability and effectiveness of the antibody.

Commonly Used Antibodies and Proteins with OX-90 (OX-7 Anti-CD90/Thy-1.1)

The clone OX-7 (often referred to as "OX-90") is a monoclonal antibody specific for CD90 (Thy-1.1), a GPI-anchored glycoprotein expressed on various cell types including thymocytes, neuronal cells, stem cells, and connective tissues. The use of OX-7 spans immunology, neuroscience, and stem cell research, and is often combined with other antibodies or proteins for multiparametric analyses.

Common Companion Antibodies and Markers

• Peripherin: In neuroscience studies, OX-7 (anti-CD90/Thy-1.1) has been used alongside antibodies against peripherin to study neurite outgrowth in sympathetic rat neurons. This combination helps distinguish neuronal subpopulations and assess neuronal differentiation or regeneration.
• Other Cell Surface Markers: In immunophenotyping (e.g., flow cytometry), OX-7 is frequently used with antibodies against other T cell markers (e.g., CD3, CD4, CD8), B cell markers (e.g., CD19, CD20), myeloid markers (e.g., CD11b, CD14), and stem cell markers (e.g., CD34, CD44, CD105). These combinations allow for the identification and isolation of specific immune or stem cell subsets.
• Conjugated Secondary Antibodies: For detection, OX-7 is often used with secondary antibodies (e.g., anti-mouse IgG) conjugated to fluorophores or enzymes (e.g., FITC, PE, APC, biotin). These enable visualization in techniques like flow cytometry, immunohistochemistry (IHC), and immunofluorescence (IF).
• Isotype Controls: In experimental controls, OX-7 may be used alongside isotype-matched irrelevant antibodies to verify specificity.

Commonly Co-detected Proteins

• Cytokines and Growth Factors: In functional assays, OX-7-positive cells (e.g., T cells, stem cells) may be analyzed for secretion or expression of cytokines (e.g., IL-2, IFN-?) or growth factors implicated in immune responses or tissue repair.
• Extracellular Matrix Proteins: In connective tissue research, OX-7 (identifying fibroblasts or mesenchymal stem cells) may be paired with antibodies against collagen, fibronectin, or other matrix components to study tissue architecture or remodeling.

Applications in Multiparametric Assays

• Flow Cytometry Panels: OX-7 is part of multiparameter panels to dissect complex cell populations, especially in thymus, spleen, lymph node, and bone marrow studies. Example panels include OX-7 + CD4 + CD8 + CD25 for T cell subsets or OX-7 + CD34 + CD45 for stem/progenitor cell identification.
• Immunohistochemistry & Immunofluorescence: In tissue sections, OX-7 staining may be combined with other lineage markers (e.g., GFAP for astrocytes, NeuN for neurons, CD31 for endothelial cells) to localize Thy-1.1-expressing cells within their tissue context.

Summary Table: Example Antibody and Protein Combinations with OX-7

Key Considerations

• Species Specificity: OX-7 reacts with rat CD90 (Thy-1.1) and certain mouse strains (e.g., AKR, FVB), but not with mouse strains expressing Thy-1.2 (e.g., CBA, BALB/c). This is crucial when designing cross-species experiments.
• Validation: Combination with validated antibodies (e.g., using orthogonal mass spectrometry or recombinant expression) ensures specificity and reliability in complex assays.

The specific choice of companion antibodies or proteins depends on the biological question, cell type, and experimental technique. However, the above combinations are commonly encountered in the literature and reflect the versatility of OX-7 in multiparametric cell and tissue analyses.

Key Findings from Clone OX-90 Citations in Scientific Literature

Clone OX-90 is a well-established rat monoclonal antibody that specifically recognizes mouse CD200 (also known as OX2), a type 1 membrane glycoprotein of the immunoglobulin superfamily. The available product documentation and technical summaries provide clear information about its applications and validated uses, but direct citations of primary scientific literature (such as peer-reviewed articles or experimental reports) detailing novel mechanistic or functional discoveries specifically attributed to clone OX-90 are notably absent from the search results.

Applications and Characteristics of Clone OX-90

• Detection and Research Use: OX-90 is widely used for detecting mouse CD200/OX2 in techniques such as Flow Cytometry, Immunohistochemistry, Immunofluorescence, ELISA, and for blocking studies. It is available in various formats, including purified and ready-to-use for flow cytometry with APC conjugation.
• Species and Isotype: The antibody is of rat origin (IgG2a, kappa), and is highly specific for mouse CD200.
• Biological Context: CD200 expression is largely restricted to thymocytes, neurons, B cells, splenic follicular dendritic cells, endothelium, and certain subsets of T cells and dendritic cells.
• Functional Role: CD200 engages with its receptor, CD200R, resulting in the inhibition or downregulation of myeloid cell activity. Blocking this interaction reduces inhibitory thresholds for these cells, suggesting a role in immune regulation.

Lack of Novel Mechanistic or Functional Discoveries

• No Primary Literature Cited: The product pages for OX-90 reference its use in research but do not cite peer-reviewed papers specifically reporting original findings generated using this clone. This suggests that while OX-90 is a reliable tool for CD200 detection, it has not been the focal point of published studies that uncovered new biology or therapies related to CD200.
• Biological Insights: The functional insights mentioned (e.g., downregulation of myeloid cell activity upon CD200–CD200R engagement) are general to the CD200 pathway, not unique to studies employing clone OX-90. These findings are typically referenced from broader CD200 literature, not from experiments using OX-90 as a primary tool.

Summary Table: OX-90 Key Facts

Conclusion

Clone OX-90 is a highly validated and widely used monoclonal antibody for the detection and study of mouse CD200/OX2 in various assay formats. However, the search results do not provide evidence of novel scientific discoveries or mechanistic insights specifically attributed to the use of clone OX-90 in peer-reviewed literature. Its primary value is as a reliable research reagent for detecting and manipulating CD200 in the mouse system, contributing to broader studies of immune regulation mediated by the CD200–CD200R axis. For in-depth, original scientific findings, one would need to consult the primary CD200 literature, as no such studies are directly cited in the available product documentation.