Anti-Mouse CD22 – Purified in vivo GOLD™ Functional Grade

In Vivo Applications of the Cy34.1 Clone in Mouse Studies

Cy34.1 is a monoclonal antibody targeting mouse CD22, a sialic acid-binding immunoglobulin-like lectin (Siglec-2) expressed primarily on mature B cells. Its use in vivo is well-documented, especially in immunology and neuroscience research.

Major Uses in In Vivo Mouse Studies

• B Cell Depletion: Cy34.1 is employed for in vivo B cell depletion, often in combination with other antibodies like anti-CD19 (clone 1D3) and anti-rat ? light chain (clone MAR 18.5). This approach allows selective removal of B cell populations from mice, enabling researchers to dissect the roles of B cells in immune responses and autoimmune diseases.
• Modulation of B Cell Function: The antibody has been shown to augment B cell proliferation in response to stimuli such as LPS or anti-mouse Ig µ chain, suggesting it can influence B cell activation pathways in vivo. This modulation is useful for studying B cell signaling and regulation, including negative feedback mechanisms that prevent autoimmunity.
• Neuroinflammation and Cognitive Studies: A unique application involves brain infusion of Cy34.1 in aging mice, where it was shown to improve cognitive function and promote clearance of neurotoxic substances, likely via modulation of microglial function (a CNS-resident immune cell population). This points to potential therapeutic applications for neurodegenerative conditions associated with chronic inflammation.

Technical Details

• Dosage and Administration: While specific dosing regimens are not detailed in the provided sources, Cy34.1 is typically administered intravenously or intraperitoneally for systemic effects, and intracerebrally for CNS-targeted studies.
• Isotype and Controls: The antibody is of mouse IgG1, ? isotype, and appropriate isotype controls should be used in experimental designs to account for non-specific effects.
• Conjugation and Detection: Unconjugated Cy34.1 is available for functional studies, while fluorochrome-conjugated versions (e.g., AF488) are used for in vivo imaging or flow cytometry post-experiment.

Biological Impact

• B Cell Biology: By targeting CD22, Cy34.1 impacts B cell adhesion, activation, and negative regulation, offering a tool to probe B cell responses in infection, autoimmunity, and cancer.
• Neuroimmunology: The observed cognitive benefits in aging mice suggest a broader role for CD22 in modulating neuroimmune interactions, beyond classical B cell biology.

Summary Table: In Vivo Applications of Cy34.1

Conclusion

Cy34.1 is a versatile tool in mouse in vivo studies, primarily used for B cell depletion and modulation, with emerging roles in neuroimmunology and cognitive research. Its applications range from basic immunology to preclinical modeling of neurodegenerative diseases.

Based on the search results, the correct storage temperature for the sterile packaged clone Cy34.1 is 2°C to 8°C (36°F to 46°F). The antibody should be stored undiluted at this temperature range and protected from prolonged exposure to light, with instructions not to freeze the product.

Storage Requirements

The clone Cy34.1, which appears to be a CD22.2 mouse anti-mouse antibody, requires specific storage conditions to maintain its stability and effectiveness. The product should be kept in a standard laboratory refrigerator within the specified temperature range, ensuring consistent cold chain maintenance.

Additional Storage Considerations

Beyond temperature control, proper storage of this clone requires protection from light exposure. The antibody should remain in its original packaging or be stored in a dark environment to prevent photodegradation. Additionally, the product must never be frozen, as this can damage the antibody structure and compromise its binding capacity.

These storage conditions are consistent across multiple manufacturers of similar CD22.2 antibody products, indicating that the 2°C to 8°C temperature range with light protection represents the optimal conditions for maintaining the biological activity of this particular clone.

Commonly Used Antibodies and Proteins in Combination with Cy34.1

Cy34.1 is a monoclonal antibody specific for mouse CD22, widely used in B cell research, especially in studies focusing on B cell development, activation, and immunoregulation. In the literature, Cy34.1 is often used in combination with other antibodies and proteins to characterize B cell subsets, investigate surface markers, and assess functional responses.

Frequent Combinations

• B220 (CD45R): A pan-B cell marker commonly used alongside Cy34.1 to identify and differentiate B cell populations by flow cytometry. B220 is expressed on all B lineage cells, while CD22 (detected by Cy34.1) is more specific to mature B cells and certain B cell subtypes.
• IgD: Often co-stained with Cy34.1 to distinguish mature follicular B cells (B220+IgD+) from other B cell subsets.
• CD23: Used with Cy34.1 to identify follicular B cells, as CD23 is expressed on a subset of mature B cells.
• BP-1 (Ly-51): Sometimes included with Cy34.1 to further delineate B cell developmental stages or subsets.

Key findings from scientific literature citing clone Cy34.1 focus on its utility in immunology research, particularly for detecting and modulating the function of CD22, a B-cell surface antigen in mice.

• Cy34.1 antibody binds specifically to mouse CD22, a glycoprotein involved in B-cell regulation, signaling, and adhesion. This makes it an important tool for studying B-cell development, activation, response to stimuli, and the pathology of diseases such as leukemia, lymphoma, and autoimmune disorders.

• Functional studies show Cy34.1’s immunomodulatory effects:

  • It can augment B-cell proliferation in response to immune stimuli such as LPS or anti-Ig µ chain, implying a role in B-cell activation.
  • The antibody has been used to explore negative regulation of B-cell activation, and protection from autoimmunity, reflecting CD22’s regulatory role.

• Detection and research applications:

  • Cy34.1 is commonly applied in flow cytometry and fluorescence microscopy for identifying and quantifying CD22 expression in various mouse strains.
  • It helps interrogate B-cell population dynamics, especially in strains expressing the Lyb-8.2 alloantigen, which include common research models like BALB/c and C57BL/6.

• Neuroimmunology roles:

  • Recent studies have used Cy34.1 to modulate CD22 in the brain, showing it can alleviate amyloid ?-induced neuroinflammation and improve cognitive function in aging mice. This finding suggests therapeutic implications for age-related neurological disorders and highlights the relevance of B-cell signaling in neurodegenerative disease models.

• Technical details:

  • Cy34.1 is a mouse IgG1, ? isotype antibody, available conjugated (AF488 for fluorescence) or unconjugated, and is recommended for specific flow cytometric protocols.
  • It reacts with mouse CD22 and does not cross-react with strains lacking the relevant CD22 epitope, ensuring specificity in experimental contexts.

• Quality control and research standardization:

  • Most suppliers test Cy34.1 lots for quality in flow cytometry, and recommend careful dilution and storage to maintain antibody stability.

These findings establish clone Cy34.1 as a standard, functionally validated monoclonal antibody for researching B-cell biology, autoimmune modulation, and emerging roles in neuroimmune interactions.