Anti-Mouse CD226 (DNAM-1) [Clone 480.1] — Purified in vivo PLATINUM™ Functional Grade

Clone 480.1 is an anti-mouse CD226 (DNAM-1) monoclonal antibody commonly used in in vivo mouse studies to functionally block or deplete CD226-expressing cells, particularly in the context of cancer research.

CD226 (also known as DNAM-1) is an important costimulatory molecule expressed on T cells and NK cells that plays a key role in immune activation and anti-tumor responses. The 480.1 clone is a rat IgG2a k antibody that specifically binds to mouse DNAM-1 and is suitable for in vivo applications due to its ultra-low endotoxin formulation.

How clone 480.1 is used in vivo mouse studies:

• Functional blockade: Researchers administer the 480.1 antibody by intraperitoneal (i.p.) or intravenous (i.v.) injection to block DNAM-1 signaling on T or NK cells, thereby assessing the functional roles of this pathway in tumor immunity and other disease models.
• Depletion studies (less common for DNAM-1): In some protocols, repeated higher dosing can lead to depletion of DNAM-1–expressing cells, though the 480.1 clone is most often used for functional blockade.
• Assessment of immune responses: Blocking CD226 with 480.1 allows investigators to determine how DNAM-1 influences anti-tumor immunity, T cell activation, surveillance mechanisms, or disease progression in various mouse models.

Key considerations:

• The 480.1 antibody is often used together with other antibodies or functional assays to dissect complex immune interactions in vivo.
• Dosing and administration route are tailored to experimental goals and mouse strain.

No relevant information was found linking clone 480.1 to other non-immunological uses or to the CLoNe lineage tracing system, which is a distinct genetic labelling tool used for tracking cell clones. The usage described here is specific to immunological modulation and not lineage tracing.

The correct storage temperature for a sterile packaged clone 480.1 is not specified in the provided search results, which focus on general biological materials and sterile devices. However, standard practice for sterile packaged biological cell clones is to store them at 2–8°C (refrigerator temperature) if they are shipped or labeled as "ready-to-use" and viable for short-term use, or at -80°C or lower (ultra-low temperature freezer) if meant for long-term storage or for maintaining viability over extended periods.

• If the packaging specifically mentions storage conditions, always follow the manufacturer's instructions on the label.
• For most sterile tissue culture reagents (like antibodies), the typical guideline is 2–8°C, protected from light, without freezing.
• For clonal cells or viable biological specimens, typical laboratory practice—if not otherwise specified—is cryopreservation at -80°C or in liquid nitrogen (below -130°C) for long-term storage, or 2–8°C for very short-term holding, such as overnight or during shipping.

If you have access to the actual product label, always use that as your primary source. If not, storing at 2–8°C is the safest general guideline for most sterile packaged biological clones intended for short-term use, and -80°C or lower for long-term viability.

If this is a plant clone, as sometimes referenced in horticultural contexts, some growers keep plant clones at 2–4°C (35–39°F) for a few weeks, but those require specific humidity and not full sterility.

The search results lack a direct reference to "clone 480.1", so inference is based on standard laboratory protocols for sterile packaged biologicals. Always verify with your supplier or the official documentation for clone 480.1, if available.

In the scientific literature, antibody 480.1 (commonly used to detect mouse CD226) is frequently used in combination with antibodies recognizing cell surface markers or proteins relevant to NK and T cell biology, immune activation, and tumor recognition. The most common combinations include antibodies targeting proteins such as CD3, CD4, CD8, CD155, CD112, NK1.1, and CD49b.

Key proteins and antibodies used with 480.1 in published studies:

• CD3: Marker for all T cells.
• CD4: Marker for helper T cells.
• CD8: Marker for cytotoxic T cells.
• NK1.1/CD49b/DX5: Markers for NK cells.
• CD155 and CD112: Ligands for CD226 (used for blocking or ligand analysis).
• CD45: General leukocyte marker (often included in gating strategies).

These antibodies are commonly used as part of multiparameter flow cytometry panels to characterize immune cell subsets, analyze cytotoxicity, or dissect receptor-ligand interactions.

Additional commonly used reagents and approaches:

• Fluorophore-conjugated secondary antibodies (e.g., PE, FITC, APC) for multiplexed cell staining.
• Viability dyes to exclude dead cells in flow cytometry.
• Blocking antibodies (for CD155/CD112 or Fc receptors).
• For functional studies, antibodies inhibiting or stimulating CD226, CD155, or CD112 are included.

The selection of antibodies depends on the experimental design—whether identifying cell types, examining receptor expression, or investigating ligand-receptor interactions in cytotoxicity assays. For advanced applications, combinations may include additional functional proteins such as therapeutic peptides, Fc-fusion proteins, or fluorescent payloads for in vivo imaging.

In summary, 480.1 is most often paired with markers for lymphocyte identification, ligands for CD226, and functional or imaging agents when broader phenotyping or mechanistic studies are undertaken.

The key scientific findings from citations of clone 480.1 focus on its central role in immunological studies, particularly in the functional analysis of DNAM-1 (CD226) in cytotoxic lymphocyte biology.

Key findings include:

• Clone 480.1 is widely cited as an anti–DNAM-1 (CD226) monoclonal antibody used in flow cytometry, cell sorting, and functional blocking assays to study the function of this receptor in immune cells.

• Studies utilizing 480.1 demonstrate that DNAM-1 is essential for the co-stimulation and activation of CD8 T cells when these cells interact with nonprofessional antigen-presenting cells. This was shown by blocking DNAM-1 with 480.1 or genetic knockout, leading to diminished T cell proliferation and interferon-gamma (IFN-?) production.

• 480.1 has been instrumental in demonstrating the requirement of DNAM-1 for effective immune synapse formation, cytotoxic activity, and cytokine production by CD8+ T cells and NK cells, especially in the context of tumor immunity and viral infection models.

• The antibody has also been used to dissect the molecular mechanisms behind CD155 (PVR)—the main ligand for DNAM-1—expression on dendritic cells and its impact on adaptive immune responses, reinforcing the importance of the DNAM-1 pathway in immune regulation.

In summary: Citations of clone 480.1 provide strong evidence for its critical role as a functional reagent that helped establish DNAM-1 as a key co-stimulatory molecule for cytotoxic lymphocytes, essential for efficient immune responses and now a frequent target in both basic research and translational immunology.