Anti-Mouse ICOSL (CD275) (Clone HK5.3) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse ICOSL (CD275) (Clone HK5.3) – Purified in vivo GOLD™ Functional Grade
Product No.: C2369
Clone HK5.3 Target B7-H2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names ICOSLG, ICOS-L, B7RP-1, LICOS, B7 homolog 2, B7-H2, B7H2, B7h Isotype Rat IgG2a Applications B , FC , in vivo , N |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen Transfected Cell Line for Mouse B7H2 Product Concentration ≥ 2.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829462 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume or 100 µL of whole blood. Additional Applications Reported In Literature ? Additional reported applications (for the relevant formats) include: Blocking ligand binding Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rat Anti-Mouse ICOSL (CD275) (Clone HK5.3) recognizes an epitope on Mouse CD275. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background ICOSL, also known as CD275 and B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains. ICOSL is expressed on B cells and macrophages. It binds to ICOS on activated T cells and thus delivers a positive costimulatory signal for optimal T cell function1. ICOS and B7RP-I do not interact with proteins in the CD28-B7 pathway2. The structural features of ICOSL are crucial for its costimulatory function. ICOSL constructs lacking either the IgC or IgV domain demonstrates that receptor binding is mediated solely by the IgV domain but requires the IgC domain for maintaining the structural integrity of the protein3. Antigen Distribution splenic B-cells, T-cells, dendritic cells and macrophages Ligand/Receptor ICOS Function Binds to ICOS. Co-stimulates T cell responses including proliferation and cytokine secretion. Stimulates B cell proliferation and differentiation to plasma cells. PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone HK5.3 is an anti-mouse ICOSL (CD275) monoclonal antibody that is widely used in in vivo mouse studies to block the interaction between ICOSL and its receptor ICOS, thereby modulating immune responses. In in vivo mouse models, HK5.3 is primarily used in the following ways:
In summary, in vivo, clone HK5.3 serves as an experimental tool to interrogate the role of ICOSL in mouse immune regulation, particularly in the study of autoimmune and inflammatory diseases through immune checkpoint modulation. HK5.3 is an antibody commonly used to target mouse ICOSL (CD275) in both in vitro and in vivo studies. In the literature, researchers often use HK5.3 in combination with other antibodies or proteins to dissect immune pathways, investigate costimulatory molecule functions, or distinguish cell types in functional experiments. Frequently used antibodies and proteins with HK5.3 include:
In the context of blocking experiments and in vivo depletion, HK5.3 is often administered alongside:
Some studies also pair recombinant ligands or Fc fusion proteins (e.g., recombinant ICOS or ICOSL-Fc) with HK5.3 to probe receptor-ligand interactions or provide costimulatory signals. These choices depend on the experimental goal (e.g., in vitro vs. in vivo immunity, cell sorting, signal inhibition), cell type, and compatibility with detection methods. Cross-reactivity, host species, and secondary antibody selection are also considered when combining HK5.3 with other reagents. If you are seeking a more targeted list of antibodies for a specific assay (e.g., flow cytometry panel design), providing that detail would allow for a more tailored response. Clone HK5.3 is a rat anti-mouse ICOSL (CD275) monoclonal antibody extensively cited in immunology literature for its ability to inhibit ICOSL function in vivo, thereby modulating T cell and B cell responses in mouse models. Key findings from scientific literature citing HK5.3 include:
In summary, clone HK5.3 is a well-validated, widely used tool antibody for functional blockade of ICOSL in mice, serving as both a benchmark and research tool for studying T cell/B cell responses and for developing new therapies targeting the ICOS-ICOSL pathway. Dosing regimens of clone HK5.3 (anti-mouse ICOSL/CD275) vary depending on the experimental application (in vitro staining vs. in vivo functional blocking/depletion) and specific mouse model, but published data and vendor guidelines provide general frameworks.
Summary Table: HK5.3 Dosing by Application
These recommendations are based on reference protocols for antibody-mediated blockade/depletion in mice. For specialized models, consult primary literature or adjust according to pilot titration data. References & Citations1. Wiendl, H. et al. (2003) Brain 126:1026 2. Yoshinaga, SK. et al. (1999) Nature 402:827 3. Chattopadhyay, KJ. et al. (2006) Immunol. 177:3920 Technical ProtocolsCertificate of Analysis |
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