Anti-Mouse CD40-BX045 (3/23); Alexa Fluor™ 647-RX045

Recombinant mouse CD40 protein

This PhenoCycler-Fusion (CODEX)® barcoded antibody is formulated in phosphate buffered saline (150 mM NaCl) PBS, EDTA pH 7.2 containing 0.09% sodium azide as a preservative. The CODEX® reporter is lyophilized and formulated in 1X Tris-EDTA (TE) pH 8.0 (10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0)

Manufactured in an animal-free facility using only in vitro cell culture techniques, purified by affinity chromatography, and conjugated to a specific barcode under optimal conditions.

CODEX® barcoded antibody - Liquid; CODEX® reporter - Lyophilized

This PhenoCycler-Fusion (CODEX)® barcoded antibody is stable when stored at 2-8°C for up to 1 year (do not freeze). The CODEX® reporter is stable when frozen at -20°C for up to 1 year.

Clone 3/23 recognizes an epitope on mouse CD40.

CD154 is expressed on activated CD4+ T cells.

CD40, also known as TNFSF5, is a type I transmembrane protein and member of the TNF receptor family. The binding of CD40L (CD154) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects₁. CD40 is expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells ₂. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells and is critical for immunoglobulin (Ig) class switching₃. The membrane-anchored CD40L is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. Some of the early intracellular signaling by the CD4-CD40L system includes the association of the CD40 with TRAFs and the activation of various kinases ₄. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of CD4-CD40L is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

PhenoCycler-Fusion (CODEX)® reporters are made up of a fluorescent dye and a short oligonucleotide DNA barcode called a PhenoCycler-Fusion (CODEX)® Tag. Fluorescent reporters enable highly specific detection of corresponding barcodes, and the use of spectrally separated dyes allow for precise signal detection in up to three distinct fluorescence channels at one time.

1. Grewall, I. S. et al. (1996) Science 73:1864
2. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569
3. Armitage, R. J. et al. (1993) J. Immunol. 150:3671
4. Pullen, S. S. et al. (1999) Biochemistry 38:10168