Anti-Mouse CD154 – FITC

Anti-Mouse CD154 – FITC

Product No.: C2823

[product_table name="All Top" skus="C2823"]

- -
- -
Clone
3/23
Target
CD154
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TNFSF5, TNFRSF5, TNF Receptor 5, Bp50, CD40
Isotype
IgG2a κ
Applications
FC

- -
- -
Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Recombinant mouse CD40 protein
Product Concentration
0.5 mg/ml
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This Fluorescein (FITC) conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (488 nm)
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 3/23 recognizes an epitope on mouse CD40.
Antigen Distribution
CD154 is expressed on activated CD4+ T cells.
Background
CD40, also known as TNFSF5, is a type I transmembrane protein and member of the TNF receptor family. The binding of CD40L (CD154) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects1. CD40 is expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells 2. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells and is critical for immunoglobulin (Ig) class switching3. The membrane-anchored CD40L is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. Some of the early intracellular signaling by the CD4-CD40L system includes the association of the CD40 with TRAFs and the activation of various kinases 4. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of CD4-CD40L is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

Antigen Details

Ligand/Receptor
CD40
NCBI Gene Bank ID

References & Citations

1. Grewall, I. S. et al. (1996) Science 73:1864
2. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569
3. Armitage, R. J. et al. (1993) J. Immunol. 150:3671
4. Pullen, S. S. et al. (1999) Biochemistry 38:10168
Flow Cytometry
- -
- -

Formats Available

- -
- -
Products are for research use only. Not for use in diagnostic or therapeutic procedures.