Anti-Mouse CD154 – FITC

Recombinant mouse CD40 protein

This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

This Fluorescein (FITC) conjugate is stable when stored at 2-8°C. Do not freeze.

Next Day 2-8°C

Blue Laser (488 nm)

Clone 3/23 recognizes an epitope on mouse CD40.

CD154 is expressed on activated CD4+ T cells.

CD40, also known as TNFSF5, is a type I transmembrane protein and member of the TNF receptor family. The binding of CD40L (CD154) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects₁. CD40 is expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells ₂. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells and is critical for immunoglobulin (Ig) class switching₃. The membrane-anchored CD40L is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. Some of the early intracellular signaling by the CD4-CD40L system includes the association of the CD40 with TRAFs and the activation of various kinases ₄. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of CD4-CD40L is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

CD40

1. Grewall, I. S. et al. (1996) Science 73:1864
2. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569
3. Armitage, R. J. et al. (1993) J. Immunol. 150:3671
4. Pullen, S. S. et al. (1999) Biochemistry 38:10168