Anti-Mouse CD40 (Clone FGK45) – Purified In vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.F1195
Clone
FGK45
Protein
CD154
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Bp50, TNFRSF5
Isotype
Rat
IgG2a
Applications
FA
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
F1195-1.0 mg
1.0 mg
$198.00
In stock
Max:
Min: 1
Step: 1
F1195-5 mg
5 mg
$355.00
In stock
Max:
Min: 1
Step: 1
F1195-25 mg
25 mg
$1,025.00
In stock
Max:
Min: 1
Step: 1
F1195-50 mg
50 mg
$1,650.00
In stock
Max:
Min: 1
Step: 1
F1195-100 mg
100 mg
$2,220.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Recombinant Mouse CD40 Fusion Protein
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone FGK45 recognizes an epitope on mouse CD40.
Antigen Distribution
CD40 is widely expressed on antigen-presenting cells such as dendritic cells, B cells, macrophages, and monocytes, in addition to non-immune endothelial cells, basal epithelial cells, and a variety of tumors.
Background
CD40, also known as TNFSF5, is a type I transmembrane protein and member of the TNF receptor family. The binding of CD40L (CD154) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects (1). CD40 is expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells (2). Signaling though CD40 plays an important role in the proliferation and differentiation of B cells and is critical for immunoglobulin (Ig) class switching (3). The membrane-anchored CD40L is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. Some of the early intracellular signaling by the CD4-CD40L system includes the association of the CD40 with TRAFs and the activation of various kinases (4). Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of CD4-CD40L is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

Antigen Details

Protein
Ligand/Receptor
CD154 (CD40L)
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Costimulatory Molecules
.
Immunology
.
Neuroscience
.
Neuroscience Cell Markers
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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