Recombinant Human CD40 Ligand (soluble)

Pricing & Details

Product. No.C386
Alternate Names
CD154, CD40LG, TRAP, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, gp39, hCD40L, CD40, sCD40L
Expression Host
E. coli
Reactivity Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
C386-25 µg
25 µg
$239.00
In stock
Max:
Min: 1
Step: 1
C386-500 µg
500 µg
$2,750.00
In stock
Max:
Min: 1
Step: 1
C386-1 mg
1 mg
$5,500.00
In stock
Max:
Min: 1
Step: 1

Data

Background

CD40 is a 48 kD type I transmembrane glycoprotein that is a member of the TNFR superfamily. CD40, in association with its ligand CD154 (CD40L) - a 39 kD protein, acts as a costimulatory molecule for the activation of B cells, dendritic cells, monocytes, and other antigen presenting cells. CD40 is involved in Ig isotype switching and dendritic cell maturation, as well as the activation, differentiation and proliferation of B cells. CD40 interacts with TNFR2 and is involved in the regulation of signal transduction. CD40 is a potential target for cancer immunotherapy. Blocking the interaction of CD40 with its ligand (CD154) is the sought-after therapeutic objective for preventing and/or improving both autoimmune diseases and transplant rejection. Studies have shown that monoclonal antibodies that block CD154 in human clinical trials resulted in unanticipated vascular complications. Hence, an interest in the therapeutic potential for antagonist mAbs specific for human CD40 is emerging. Antibodies of particular therapeutic interest are those that do not inhibit CD40 signaling via physical competition with CD154. Additionally, the interaction of CD40 and its ligand (CD154) is found to be essential for amyloid-beta-induced microglial activation, thus plays a significant part in Alzheimer disease pathogenesis.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human sCD40L was determined by it ability to stimulate proliferation of enriched human B cells in the presence of 20 ng/ml of recombinant human IL-4. The expected ED50 for this effect is typically 10 - 30 ng/ml in the presence of 10 µg/ml of a cross-linking antibody PN:P246.
Fusion Protein Tag
Histidine Tag
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
hhhhhhensf emqkgdqnpq iaahviseas skttsvlqwa ekgyytmsnn lvtlengkql tvkrqglyyi yaqvtfcsnr eassqapfia slclkspgrf erillraant hssakpcgqq sihlggvfel qpgasvfvnv tdpsqvshgt gftsfgllkl
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CD40 Ligand is Mr 17.7 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 19 kDa (reducing conditions).
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution of 20 mM PO4, 200 mM NaCl and 0.1 mM EDTA.
Storage and Stability
This lyophilized protein is stable for up to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution and addition of a carrier protein, this cytokine may be stored at 2°C to 8°C for one month or for three months at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Schönbeck, U. et al. (2006) Clin Sci (Lond).111(5):341-7. Article Link
2. Schönbeck, U. et al. (2005) Diab Vasc Dis Res. 2(2):81-7. Article Link
3. Ferran, C. et al. (2003) Circulation.108(9):1113-8. Article Link
4. Zhang, G. et al. (2004) Curr. Opin. Struct. Biol. 14:154
5. Lievens, D. et al. (2009) Thromb. Haemost. 102:206
6. Schönbeck, U. et al. (2001) Cell. Mol. Life Sci. 58:4
7. Fanslow, WC et al. (1994) Sem. Immunol. 6:267
8. Bhushan, A. et al. (2002) Immunol. Res. 24:311
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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