Anti-Mouse/Human CD49d (Clone PS/2) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C798
Clone
PS/2
Protein
CD49D
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
VLA-4α, ITGA4, Integrin α4
Isotype
Rat
IgG2b κ
Applications
FA
,
FC
,
IHC
,
IP
Prod No.
Size
Price
Avail.
Qty
Add to cart
C798-1.0mg
1.0 mg
$225.00
In stock
Max:
Min: 1
Step: 1
C798-5.0mg
5.0 mg
$445.00
In stock
Max:
Min: 1
Step: 1
C798-25mg
25 mg
$1,285.00
In stock
Max:
Min: 1
Step: 1
C798-50mg
50 mg
$2,045.00
In stock
Max:
Min: 1
Step: 1
C798-100mg
100 mg
$2,800.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse/Human
Host Species
Rat
Immunogen
P815 DBA/2 murine mastocytoma cells.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUMTM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC
Other Applications Reported In Literature ?
FA, IHC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
PS/2 activity is directed against mouse CD49d and is cross reactive against human CD49d.
Antigen Distribution
CD49d is expressed on T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.
Background
Integrins are a large family of heterodimeric transmembrane molecules that mediate adhesion, migration, cell survival, and cell differentiation. CD49d is a single-pass type I membrane glycoprotein also known as integrin alpha-4 (Uniprot Accession P13612). CD49d is the α4 subunit of integrin heterodimers alpha-4/beta-1 (VLA-4; CD49d/CD29; α4β1 integrin) and alph-4/beta-7 (LPAM-1)1. These integrins act as receptors for fibronectin and VCAM1 (CD106). Integrin alpha-4/beta-7 is also a receptor for MADCAM1.

CD49d is expressed on most lymphocytes, granulocytes, monocytes, and thymocytes. CD49d/CD29 (VLA-4; α4β1) is expressed at high levels on the surface of lymphohematopoietic progenitors and is involved in their development and proliferation. CD49d/CD29 integrin/VCAM-1 interactions facilitate B cell adhesion to stromal cells and enhance B cell activation. In the absence of alpha-4 integrins, pre-B cells fail to transmigrate and proliferate.

PS/2 recognizes murine and human CD49d2. PS/2 was generated by immunizing Fisher rats with P815 cells and subsequently fusing the spleen cells with Sp2/0. Hybridoma supernatants were screened by cell adhesion assay and cells producing blocking antibodies were cloned. Adhesion is blocked in a dose dependent manner when PS/2 is used with P815 and +/+ 2.4 stromal cells. 70Z/3 cells are also sensitive to PS/2 inhibition. PS/2 is known to block binding of CD49d to its ligands3. Lymphocyte production is completely blocked when PS/2 is included in Whitlock-Witte culture2. PS/2 is IgG2b κ.

Antigen Details

Protein
Ligand/Receptor
Fibronectin, VCAM-1, MAdCAM-1
Function
Lymphocyte migration, T cell activation, stem cell differentiation.
PubMed
Research Area
Cell Adhesion
.
Cell Biology
.
Immunology
.
Innate Immunity

References & Citations

1. Holzmann B, Weissman IL. EMBO J. 8(6):1735-1741. 1989.
2. Miyake K, Weissman IL, Greenberger JS, et al. J Exp Med. 173(3):599-607. 1991.
3. Andrew DP, Berlin C, Honda S, et al. J Immunol. 153(9):3847-3861. 1994.
4. Miyake K, Medina K, Ishihara K, et al. J Cell Biol. 114(3):557-565. 1991.
5. Enghofer M, Bojunga J, Ludwig R, et al. Am J Physiol. 274(5):E928-E935. 1998.
6. Hokibara S, Takamoto M, Isobe M, et al. Clin Exp Immunol. 114(2):236-244. 1998.
7. Fukuoka M, Fukudome K, Yamashita Y, et al. Blood. 96(13):4267-4275. 2000.
8. Omenetti S, Brogi M, Goodman WA, et al. Cell Mol Gastroenterol Hepatol. 1(4):406-419. 2015.
9. Chung KJ, Chatzigeorgiou A, Economopoulou M, et al. Nat Immunol. 18(6):654-664. 2017.
10. Tanneau GM, Hibrand-Saint Oyant L, Chevaleyre CC, et al. J Histochem Cytochem. 47(12):1581-1592. 1999.
11. Tchilian EZ, Owen JJ, Jenkinson EJ. Immunology. 92(3):321-327. 1997.
12. Liu ZJ, Tanaka Y, Fujimoto H, et al. J Immunol. 163(9):4901-4908. 1999.
13. Bellingan GJ, Xu P, Cooksley H, et al. J Exp Med. 196(11):1515-1521. 2002.
14. Bowden RA, Ding ZM, Donnachie EM, et al. Circ Res. 90(5):562-569. 2002.
15. Hirata T, Furie BC, Furie B. J Immunol. 169(8):4307-4313. 2002.
16. Maus UA, Srivastava M, Paton JC, et al. J Immunol. 173(2):1307-1312. 2004.
17. Eshghi S, Vogelezang MG, Hynes RO, et al. J Cell Biol. 177(5):871-880. 2007.
18. Li W, Ishihara K, Yokota T, et al. Glycobiology. 18(1):114-124. 2008.
19. Vaz R, Martins GG, Thorsteinsdóttir S, et al. Cell Tissue Res. 348(3):569-578. 2012.
20. Zhang Y, Chen YC, Krummel MF, et al. J Immunol. 189(8):3914-3924. 2012.
21. Sens C, Altrock E, Rau K, et al. J Bone Miner Res. 32(1):70-81. 2017.
Flow Cytometry
IHC
Immunoprecipitation Protocol

Formats Available

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