Anti-Mouse CD5 (Lyt-1) – PE

Anti-Mouse CD5 (Lyt-1) – PE

Product No.: C3125

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CD5 (Lyt-1)
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Alternate Names
LY-1, T1, Tp67
Rat IgG2a κ

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Antibody Details

Product Details

Reactive Species
Host Species
Mouse thymus or spleen
Product Concentration
0.2 mg/ml
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
State of Matter
Storage and Handling
This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze.
Regulatory Status
Research Use Only
Country of Origin
2 – 8° C Wet Ice
Excitation Laser
Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Applications Reported In Literature ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


53-7.3 activity is directed against mouse CD5 (Lyt-1).
Antigen Distribution
CD5 (Lyt-1) is a marker used to identify T cells, B1-a cells, B cell chronic lymphocytic leukemia cells, regulatory T cell (Tregs), regulatory B cells (IL-10 producing Bregs), and the earliest thymic progenitors. CD5 is also expressed on all lymphocytes except natural killer cells.
CD5 (Lyt-1) is fundamental to immune homeostasis and immune tolerance, with many functions in T and B cells1. CD5 acts as a modulator of T and B cell receptor signaling as well as a cell survival receptor in T and B cells. CD5 is also involved in thymocyte selection, T cell effector differentiation, induces regulatory T cells in the periphery, and modulates Th17 and Th2 differentiation as well as dendritic cell function. Because of its role as an immune checkpoint modulator, CD5 is a target of immunotherapy in the treatment of cancer, autoimmune disease, and infection.

53-7.3 was generated by immunizing LOU/Ws1/M rats with mouse (SJL/J or C3H/HeJ) spleen cells or thymocyte membranes2. Spleen cells were subsequently fused with mouse myeloma NS-1 to produce a hybridoma. The antigen target CD5 (Lyt-1) was confirmed by comparative immunoprecipitation as well as blocking experiments using conventional alloantisera.

Antigen Details

CD72, gp35-37
NCBI Gene Bank ID
Research Area
Cell Biology

References & Citations

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6. Hollander N, Pillemer E, Weissman IL. Proc Natl Acad Sci U S A. 78(2):1148-1151. 1981.
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26. Soukup AA, Zheng Y, Mehta C, et al. J Clin Invest. 129(3):1180-1192. 2019.
27. Ercoli G, Ramos-Sevillano E, Nakajima R, et al. Front Immunol. 11:611661. 2021.
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29. Pastrana-Otero I, Majumdar S, Gilchrist AE, et al. Analyst. 145(21):7030-7039. 2020.
30. Ngo MT, Barnhouse VR, Gilchrist AE, et al. Adv Funct Mater. 31(51):2101541. 2021.
31. Gilchrist AE, Serrano JF, Ngo MT, et al. Acta Biomater. 131:138-148. 2021.
32. Park JS, Lee D, Yang S, et al. J Transl Med. 20(1):85. 2022.
33. Xu L, Wei C, Chen Y, et al. Nat Commun. 13(1):6881. 2022.
34. Azam Z, Sapra L, Baghel K, et al. Cells. 12(2):216. 2023.
Flow Cytometry
Immunoprecipitation Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.