Anti-Mouse CD70 (Clone TAN1-7) – Purified in vivo PLATINUM™ Functional Grade

Clone TAN1-7 is a monoclonal antibody used in in vivo mouse studies to target and block mouse CD70, a cytokine expressed on activated T cells, B cells, and dendritic cells. This clone is typically administered to mice to study the immunological roles of the CD70-CD27 pathway, which is essential for B and T cell activation as well as immune regulation.

Key uses and details:

• Function: TAN1-7 binds specifically to mouse CD70, blocking its interaction with CD27, thereby modulating immune responses, such as T and B cell activation.
• Research applications: It is commonly used to investigate the role of CD70 in immune responses, immunopathology, tumor immunity (e.g., in non-small cell lung cancer models), and the regulation of B cell activation and natural killer cell function.
• Administration: Provided as a low-endotoxin, carrier-free, and azide-free antibody suitable for direct administration (in vivo) in mouse models.
• Resulting effects: The antibody allows researchers to assess how blocking CD70 affects immune cell signaling, survivability of tumor cells, immunoglobulin synthesis, and overall immune system behavior during health and disease.

CD70 and its interaction with CD27 are crucial in adaptive immunity, so TAN1-7 is a valuable tool in dissecting these pathways in mouse models. Experimental protocols may involve single or repeated dosing, with readouts including changes in lymphocyte populations, cytokine production, or tumor progression, depending on study design.

The correct storage temperature for sterile packaged plant clones, such as TAN1-7, is generally between 2°C and 8°C (35°F to 46°F), which is standard for sterile and tissue culture plant materials. This temperature range preserves viability and sterility while minimizing metabolic activity and contamination risks.

• For sterile, packaged, or tissue culture plant clones, refrigeration at 2–8°C is standard practice in both research and commercial settings.
• The package should be protected from light if indicated by the manufacturer, and never frozen, as freezing can cause cellular damage to plant tissues.
• If you have manufacturer’s documentation (e.g., a Certificate of Analysis or product insert for TAN1-7), always follow those instructions as some clones may have specific requirements.

If TAN1-7 is a tissue culture clone or similar to those referenced for plant storage, 2–8°C is the expected standard. If this clone is a different type of biological (e.g., a vaccine or other reagent), this same range often applies, but always verify with product-specific literature for absolute certainty. If your sterile package is plant tissue culture, do not store below 2°C or above 8°C unless the supplier provides different instructions.

Commonly used antibodies or proteins with TAN1-7 are not directly identified in the available search results; it is possible that "TAN1-7" refers to a specific antibody, epitope, or reagent not widely indexed or requires precise context (such as the target antigen or field—immunology, cell biology, yeast genetics, etc.). If you meant the yeast gene/protein TAN1, literature frequently uses antibodies against protein tags (e.g., GST, His-tag) or co-purified partners for detection and analysis. If TAN1-7 refers to an antibody clone, related studies typically employ standard immune markers or antibodies targeting:

• Epitope tags (e.g., anti-GST, anti-His if the protein is tagged).
• Other structural or signaling proteins in immune studies (e.g., CD markers, cytokines, or proteins relevant to the pathway of interest, such as IFN?, TNF, and IL-6 in immunological contexts).
• Housekeeping proteins (for controls, such as actin or tubulin) in cell biological applications.

If your question was about a specific application (e.g., TAN1 in tRNA modification or as an immunological marker), please specify the context for a more targeted and accurate list of commonly used antibodies or proteins. The most explicit antibody directly cited alongside TAN1 in the search results is anti-GST.

The key findings from scientific literature regarding clone TAN1-7 citations appear to be limited or ambiguous: there is no direct match in major databases for a "clone TAN1-7." However, the search results identify two relevant, but distinct, research contexts frequently associated with similar names—Saccharomyces cerevisiae TAN1 (tRNA modification enzyme) and Sorghum Tannin1 (Tan1, a gene involved in tannin biosynthesis):

• Saccharomyces cerevisiae TAN1:

  • The TAN1 gene in yeast is required for the formation of the modified tRNA nucleoside N4-acetylcytidine (ac4C).
  • Disruption of TAN1 leads to loss of this modification, impacting growth in certain strain backgrounds, suggesting its essential role in tRNA modification and potentially broader cellular functions.
  • Restoration of TAN1 via plasmid complementation (such as a clone bearing TAN1) rescues the biochemical defect, confirming TAN1's enzymatic function in ac4C formation in tRNA.

• Sorghum Tannin1 (Tan1) gene:

  • The Tan1 gene was cloned and shown to encode a WD40 protein.
  • Natural allelic variation in Tan1 leads to frame shifts and protein truncation, causing loss of grain tannins—a trait of agronomic and nutritional interest.
  • Mapping, association, and transgenic complementation studies confirm Tan1’s role as a major determinant of tannin presence in sorghum, facilitating targeted breeding and biotechnology applications.

• No evidence of a TAN1-7 clone: None of these findings directly reference a clone called "TAN1-7." In yeast, TAN1 is the gene discussed; in sorghum, the gene designation is Tan1 or Tannin1. If "clone TAN1-7" refers to a specific construct, expression clone, or allelic variant, this information is not present in cited results, and further clarification or more targeted literature search would be necessary.

• Scope of findings: Both TAN1 (in yeast) and Tan1 (in sorghum) represent functionally pivotal genes:

  • In yeast, TAN1 is essential for a specific tRNA modification.
  • In sorghum, Tan1 governs tannin biosynthesis by encoding a structural protein with regulatory impact on metabolite accumulation.

If your inquiry is about a specific clone or construct named "TAN1-7" in a different organism or context, detail or accession information is required for a precise answer. Otherwise, literature mainly discusses TAN1/Tan1 genes as described above.