Anti-Mouse CD70 (Clone TAN1-7) – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse CD70 (Clone TAN1-7) – Purified in vivo PLATINUM™ Functional Grade

Product No.: I-2025

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Clone
TAN1-7
Target
CD70
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD27 Ligand, TNFSF7, Tumor Necrosis Factor Ligand 8A, CD27L, CD70 Antigen, CD27LG, Surface Antigen CD70, CD70 Molecule, Ki-24 Antigen; CD70 Antigen; TNLG8A
Isotype
IgG2a κ
Applications
B
,
ELISA
,
FA
,
FC
,
IF
,
in vivo

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Immunogen
Recombinant mouse CD70 (extracellular domain, aa residues 41-195)
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM<sup>TM</sup> antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC
ELISA
Additional Applications Reported In Literature ?
B
IF
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone TAN1-7 monoclonal antibody recognizes a direct epitope on mouse CD70 and capable of blocking the CD70-CD27 interaction involved in B and T cell activation.
Background
CD70 antibody, TAN1-7, recognizes CD70, a type II membrane protein and member of the TNF family. CD70 is the ligand for the T cell costimulatory receptor CD27 (also known as TNFRSF27)1 and is expressed on activated T cells, B cells, and dendritic cells (DCs)1,2. Ligation of CD27 on T cells with CD70 on DCs provides costimulatory signals and promotes CD4 and CD8 T cell activation3, differentiation4, and survival5,6. CD70 also regulates B cell activation and antibody production7 and enhances natural killer (NK) cell survival and function8. CD70 is constitutively expressed in several hematological and solid tumors and increases the proliferation and survival of tumor cells and regulatory T cells (Tregs), suggesting it may be an effective therapeutic target9-13. Increased levels of CD70 are also associated with autoimmune disorders, including rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE)14,15.
Antigen Distribution
CD70 is expressed on activated T cells, B cells, and dendritic cells.

Antigen Details

PubMed
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
.
Immunology

References & Citations

1. R. A. van Lier., et al. (1997) J. Immunol. 159(10):4959-65
2. R. A. van Lier. et al. (2003) J. Immunol. 170(1):33-40
3. Hintzen, R. Q. et al. (1995) J. Immunol. 154, 2612–2623
4. Soares, H. et al. (2007) J. Exp. Med. 204, 1095–1106
5. Hendriks, J. et al. (2000) Nat. Immunol.1, 433–440
6. Hendriks, J., Xiao, Y. & Borst, J. (2003) J. Exp. Med.198, 1369–1380
7. van Oers MH., et al. (2004) J Immunol. 15;173(6):3901-8. 8. Ochsenbein AF., et al. (2017) 20;130(3):297-309
9. Ansell SM., et al. (2007) Blood. 110:2537–2544
10. Doronina SO., et al. (2006) Cancer Res. 66:2328–2337
11. Weller M., et al. (2002) Cancer Res. 62:2592–2599
12. Held-Feindt, J. & R. Mentlein. (2002) Int. J. Cancer 98:352
13. Chiodi F., et al. (2005) Exp Hematol. 33:1500–1507
14. Han, B. K. et al. (2005) Lupus. 14, 598–606
15. Park, J. K. et al. (2014) Rheumatology. 53, 1896–1900
B
Indirect Elisa Protocol
FA
Flow Cytometry
IF
in vivo Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.