Anti-Mouse CD80 (B7-1) [Clone RM80] — Purified in vivo GOLD™ Functional Grade

Clone RM80 is a rat monoclonal antibody specific for mouse CD80 (B7-1) and is most commonly used in vivo for CD80 blockade in mouse models.

Common in vivo applications in mice include:

• Blocking CD80-mediated costimulatory signals: RM80 is used to functionally inhibit CD80, thereby interfering with its interaction with CD28 and CTLA-4 on T cells. This modulates immune responses, especially in studies of T cell activation, transplantation immunity, autoimmunity, or tumor immunology.
• Immunotherapy research: By blocking CD80, researchers can evaluate the dependence of certain immune or tumor responses on CD80-mediated costimulation, which is critical for dissecting the mechanisms of immune activation or tolerance in mouse disease models.

Key contexts for in vivo use:

• Functional immunological studies where the aim is to determine the role of CD80 in T cell stimulation (via CD28) versus inhibition (via CTLA-4).
• Tumor models and autoimmunity models in mice, where blockade of CD80 helps elucidate the contribution of costimulatory pathways in disease progression or therapy response.

Technical details:

• RM80 is administered via injections in mice (typically at concentrations optimized for effective blockade), and immune readouts such as T cell proliferation, cytokine production, or disease markers are measured downstream.

Summary Table:

No mainstream references indicate alternative major in vivo applications of RM80 beyond CD80 blockade and immune modulation in mice. If you require specific dosing protocols or in-depth model-specific information, those are typically reported in individual experimental publications.

Based on the search results provided, there is no information available about "RM80" or antibodies/proteins commonly used with it in the literature. The search results contain information about various other molecules and antibodies, including:

• P80: An 80 kDa antigen associated with cell proliferation in malignant cells
• CD80: A costimulatory molecule involved in T-cell activation and immune checkpoint regulation
• Various CD80-related therapeutics such as belatacept, abatacept, and davoceticept (ALPN-202)

However, none of these results discuss "RM80" specifically or mention antibodies/proteins used in conjunction with it. Without relevant search results or existing knowledge about RM80, I cannot provide information about its commonly associated antibodies or proteins in the literature.

If you could provide additional context about what RM80 refers to (such as whether it's a specific antibody clone, a protein marker, or a reagent), that would help in finding more relevant information about molecules used alongside it in research applications.

The key findings from scientific literature citing clone RM80 focus on its use as a monoclonal antibody against mouse CD45R (B220), a widely used marker for B lymphocyte identification and characterization in immunology research.

Key points from the literature include:

• Immunophenotyping and Cell Sorting: Clone RM80 is used extensively to label B220, the CD45R isoform predominantly expressed on B cells in mouse models, supporting both flow cytometric and immunohistochemical studies of B cell development, populations, and function.
• Immunohistochemistry and Tissue Analysis: This antibody allows for the detection of B-cell subsets in fixed tissues, facilitating studies on tissue distribution and roles of B cells in immune responses and disease models, including autoimmune disease and neoplasia.
• References and Validation: The antibody is cited in foundational work on hematopoietic stem cell purification, T-cell and B-cell subset identification, and inflammation or immune cell tracking in various mouse models. Notable referenced studies include:
  • Spangrude et al. (1988) on isolation of murine hematopoietic stem cells.
  • Whiteland et al. (1995) on leukocyte subset detection in paraffin-embedded tissues.
  • Rosmalen et al. (2000) on macrophage and dendritic cell analysis in diabetic mouse pancreas.

The RM80 clone is considered a validated and reliable marker antibody in the field, being cited across multiple peer-reviewed immunology studies to dissect B cell biology and immune system architecture in mice. No direct evidence was found of RM80 being linked to genetic or molecular cloning outside antibody generation/application, and the citations do not indicate use outside the B cell marker context.

Dosing regimens of clone RM80 (rat anti-mouse CD80 monoclonal antibody) in different mouse models are not comprehensively detailed in the primary reference materials available, but one specific protocol provides relevant information: in an allogeneic murine hepatocyte rejection model, mice received intraperitoneal administration of anti-CD80 antibody (clone RM80) at 100 μg/day for 5 days, starting at the time of hepatocyte transplantation.

Key dosing details from published models:

• Dosage: 100 μg per day
• Route: Intraperitoneal injection
• Duration: 5 consecutive days
• Initiation: Dosing begins concurrently with the experimental procedure (e.g., cell transplantation).

Additional context and limitations:

• The only explicit dosing protocol in the available search results refers to an immune rejection model using allogeneic hepatocyte transplantation. No comparable published dosing regimens for clone RM80 are described in oncology, infection, or autoimmunity models in the search results.
• The cited protocol combined anti-CD80 (clone RM80) with anti-CD86 antibody, possibly indicating that RM80 is used in combination regimens depending on the immune mechanism under investigation.
• Product datasheets for clone RM80 specify only the form, isotype, and target (mouse CD80/B7-1), not dosing recommendations; dosing is typically defined empirically by experimental context.
• If other mouse model types (e.g., tumor, infectious, or autoimmune models) are of interest, dosing may vary depending on disease, route (intravenous, intraperitoneal, intratumoral), or mouse strain, but such details are not specified in the retrieved sources.

Summary Table: Example RM80 Dosing Regimen

If further detail or model-specific regimens are required, consultation of primary immunological literature involving anti-CD80 (clone RM80) or direct inquiry with antibody suppliers may be necessary given the lack of broad comparative data in search results.