Anti-Mouse CD8a (Ly 2.2) [Clone 2.43] — Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD8a (Ly 2.2) [Clone 2.43] — Purified in vivo PLATINUM™ Functional Grade
Product No.: C2837
Clone 2.43 Target CD8a Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Ly-2, Ly-35, Ly-B, Lyt-2, Lyt2, Ly 2.2 Isotype Rat IgG2b Applications Depletion , FA , FC , ICC , IF Staining , IHC FFPE , in vivo , IP |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Mouse CTL clone L3 Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional GOLD™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829595 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 2.43 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? Depletion FA Additional Reported Applications For Relevant Conjugates ? ICC IF Staining IHC (Paraffin) IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 2.43 recognizes an epitope on mouse CD8a. Background CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes. Antigen Distribution CD8a is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic-cells. NCBI Gene Bank ID UniProt.org Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone 2.43 is a rat anti-mouse CD8? monoclonal antibody used in in vivo mouse studies to selectively deplete CD8+ T cells. Researchers administer clone 2.43 to mice, most commonly via intraperitoneal or intravenous injection, at doses typically ranging from 100??g to 500??g per mouse, with 250??g per mouse frequently used as a standard for effective depletion. Context and Key Details:
Advantages:
Potential Limitations:
Additional Applications:
In summary, clone 2.43 is the gold standard antibody for in vivo depletion of CD8+ T cells in mouse models, crucial for dissecting CD8+ T cell contributions in immunological research. The correct storage temperature for sterile packaged clone 2.43 (anti-mouse CD8? monoclonal antibody) depends on the intended storage duration:
For all durations:
Summary Table: Storage Duration vs. Temperature
Always consult the product datasheet or supplier for specific recommendations about your exact formulation and packaging. Commonly used antibodies or proteins with 2.43 (anti-mouse CD8?, clone 2.43) in the literature are typically selected to identify and characterize different leukocyte populations or functional states in mouse immunology experiments. The most relevant co-used antibodies/proteins include:
Other reagents frequently employed:
Common applications in which these markers are used together include:
Panel design and antibody choice depend on experimental goals, such as distinguishing T cell subsets, characterizing effector/memory populations, or confirming depletion efficacy. Summary table: Key antibodies and their use with 2.43
This structure reflects the typical experimental design in mouse immunology where clone 2.43 is used. Clone 2.43 is a monoclonal antibody widely cited in scientific literature for its ability to deplete CD8+ T cells in vivo, mainly in mouse models. Key findings from studies using clone 2.43 include:
Representative applications and findings:
Caveats and limitations:
In summary, clone 2.43 is a standard tool to dissect the function of CD8+ T cells in diverse in vivo studies, with findings relying on its specificity and effectiveness for targeted T cell depletion. References & Citations1.) Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668. PubMed 2.) Hawman DW, et al. (2021) Microorganisms 9(2):279 Journal Link Technical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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C1664 | |
C297 | |
C298 | |
C300 | |
C299 | |
C301 | |
C1644 | |
C1646 | |
C1648 | |
C1650 | |
C1645 | |
C380 | |
C2837 |
