Anti-Mouse CD8a (Ly 2.2) [Clone 2.43] — Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD8a (Ly 2.2) [Clone 2.43] — Purified in vivo PLATINUM™ Functional Grade
Product No.: C2837
Clone 2.43 Target CD8a Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Ly-2, Ly-35, Ly-B, Lyt-2, Lyt2, Ly 2.2 Isotype Rat IgG2b Applications Depletion , FA , FC , ICC , IF Staining , IHC FFPE , in vivo , IP |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Mouse CTL clone L3 Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional GOLD™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829595 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 2.43 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? Depletion FA Additional Reported Applications For Relevant Conjugates ? ICC IF Staining IHC (Paraffin) IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 2.43 recognizes an epitope on mouse CD8a. Background CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes. Antigen Distribution CD8a is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic-cells. NCBI Gene Bank ID UniProt.org Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone 2.43 is a rat anti-mouse monoclonal antibody that specifically targets CD8α, making it the gold standard for in vivo depletion of CD8⁺ T cells in mouse models. Its common applications include:
Technical context:
Other research/diagnostic uses (beyond depletion):
In summary, in vivo depletion of CD8⁺ T cells to study immune cell function and disease mechanisms is the overwhelmingly predominant use of clone 2.43 in mice. The 2.43 antibody (rat monoclonal, anti-mouse CD8a) is commonly used for depletion, staining, and functional studies of CD8+ T cells. In published literature, it is often used in combination with the following antibodies and proteins:
These combinations are especially frequent in:
Key pairs used with 2.43 in literature:
References support frequent pairing with anti-CD4 (clone GK1.5), and combining with anti-CD3 and other anti-CD8 clones in flow cytometry or depletion protocols. In summary, the antibodies most commonly used with 2.43 are anti-CD4 (GK1.5), anti-CD3, and alternative anti-CD8 clones for lineage and functional analysis in mouse immunology. Key Findings from Clone 2.43 Citations in Scientific LiteratureAnti-mouse CD8a (clone 2.43) is a highly cited monoclonal antibody in immunology research, particularly for in vivo CD8+ T cell depletion studies. Its primary uses and findings in the scientific literature include the following: Function and Applications
Advantages
Limitations and Considerations
Mechanistic and Translational Insights
Representative Experimental UseClone 2.43 has been used to deplete CD8+ T cells in vivo to assess the contribution of these cells to adaptive immunity, viral clearance, and immunopathology in various infection and vaccination models. Such studies often pair clone 2.43 with complementary reagents to dissect complex immune interactions. Summary Table: Clone 2.43 in Scientific Literature
ConclusionClone 2.43 is a cornerstone reagent in immunology for in vivo CD8+ T cell depletion, offering high specificity, established efficacy, and broad applicability across preclinical models. Its use has advanced understanding of CD8+ T cell biology in health and disease, though researchers must remain aware of potential limitations related to off-target effects and immunogenicity in long-term studies. Dosing regimens of clone 2.43 (anti-CD8α monoclonal antibody) typically range from 100 μg to 500 μg per mouse per injection, with most studies using 250 μg administered intraperitoneally or intravenously every 2–7 days, but the exact dose and schedule can be adapted to the mouse strain, disease model, desired depletion kinetics, and experimental length. Key details on regimen variation:
In summary, dosing regimens for clone 2.43 are not fixed across mouse models but are optimized (100–500 μg, usually 250 μg per mouse, every 2–7 days intraperitoneally) according to the strain, experimental timeline, and the specific biological context, with the standard protocol adaptable for disease, immune kinetics, and individual animal variation. References & Citations1.) Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668. PubMed 2.) Hawman DW, et al. (2021) Microorganisms 9(2):279 Journal Link Technical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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C1664 | |
C297 | |
C298 | |
C300 | |
C299 | |
C301 | |
C1644 | |
C1646 | |
C1648 | |
C1650 | |
C1645 | |
C380 | |
C2837 |
