Anti-Mouse/Rat CD90.1 (Thy-1.1) [Clone OX-7] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse/Rat CD90.1 (Thy-1.1) [Clone OX-7] — Purified in vivo GOLD™ Functional Grade
Product No.: C851
Clone OX-7 Target CD90.1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD90, CD90.1, Thy-1, Thy1.1, Thy1.2, Thy-1.2 Isotype Mouse IgG1 Applications FC , IF , IHC |
Antibody DetailsProduct DetailsReactive Species Mouse ⋅ Rat Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen Rat thymocyte Thy-1 antigen Product Concentration ≥ 5.0 mg/ml Endotoxin Level <1.0 EU/µg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.0-7.5, 0.005% pS80 stabilizing buffer, with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Additional Applications Reported In Literature ? FC IF FA IHC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone OX-7 activity is directed against both rat and murine CD90/Thy-1. Background CD90/Thy-1 (murine CD90.1/Thy-1.1) is a cell surface glycoprotein that was the first protein of the immunoglobulin superfamily to be discovered5. The 25 kDa core protein is N-glycosylated at three sites, leading to isoforms with a range of molecular masses (25-37 kDa). CD90 is composed of a single V-like immunoglobulin domain anchored by a disulfide bond between Cys 28 and Cys 104. Despite its lack of an intracellular domain, CD90 is located in the outer leaflet of lipid rafts at the cell membrane, allowing signaling functions by cis- and trans-interactions with a variety of proteins, including G inhibitory proteins, the Src family kinase (SFK) members src and c-fyn, and tubulin.
CD90 has been found to play a role in numerous cellular activities, such as cell adhesion, apoptosis, metastasis, inflammation, and fibrosis. In mouse strains expressing CD90.1, it is expressed on early-stage hematopoietic cells in bone marrow, thymocytes, and circulating mature T cells. The OX-7 antibody has been reported to induce leukocyte activation, glomerular nephritis, apoptosis in glomerular mesangial cells, and vascular permeability. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma. Antigen Distribution CD90 is present on a variety of cell types in murine and rat, including lymphatic vessels1, T cells2, neurons3 and fibroblasts4. Function The function of Thy1 has not been fully elucidated but is thought to play roles in regulation of cell adhesion, apoptosis, metastasis, inflammation, and fibrosis NCBI Gene Bank ID UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Common In Vivo Applications of Clone OX-7 in MiceCellular Labeling and Lineage Tracking Induction of Glomerulonephritis Models Functional Modulation of CD90.1-Positive Cells Therapeutic and Drug Delivery Applications Summary Table
Key Considerations
In summary, the primary in vivo applications of clone OX-7 in mice are cellular labeling, lineage tracking, and functional modulation of CD90.1-positive cells, with potential for disease modeling and targeted therapy in strains that express the appropriate antigen. Based on the search results, OX-7 (anti-CD90/Thy1.1 antibody) is commonly used in research alongside several other antibodies and proteins, depending on the experimental context: Structural Reference ProteinsBeta tubulin serves as a classic companion for OX-7 in immunocytochemistry (ICC) experiments, where it functions as a structural reference marker and provides normalization for imaging studies. Secondary Detection AntibodiesAnti-mouse IgG secondary antibodies are routinely employed with OX-7, since OX-7 is a mouse monoclonal antibody of the IgG1 isotype. These secondary antibodies enable indirect detection and visualization of OX-7 binding in various immunological assays. Cell Marking and VisualizationIn studies examining neuronal cells, OX-7 has been used in conjunction with peripherin staining to visualize sympathetic rat neurons through fluorescence microscopy. This combination allows researchers to assess neurite outgrowth, demonstrating OX-7's ability to promote neuronal development when combined with peripherin as a neuronal marker. Complement ComponentsIn models studying antibody-mediated cytotoxicity, OX-7 is examined alongside C3 complement protein to investigate complement-dependent cytotoxicity mechanisms. Research has utilized this combination to examine tissues including the kidney, adrenal gland, and thymus in rat anti-Thy-1 models. The specific antibodies and proteins used with OX-7 ultimately depend on the experimental system, with the choice driven by whether researchers are conducting flow cytometry, microscopy, functional assays, or complement-mediated studies. The OX-7 clone is a widely used rat monoclonal antibody targeting CD90 (Thy-1), and key findings from its citations in scientific literature emphasize its significant roles in cell biology and neuroscience. Key findings include:
Summary Table: Major OX-7 Findings
These attributes have established OX-7 as a standard tool in research on stem cells, immunology, and neuroscience. Dosing regimens of clone OX-7 vary significantly across different mouse models due to fundamental differences in CD90 (Thy-1) allelic variants expressed by different strains, as well as the specific experimental application being pursued. The variability in dosing protocols stems from the polymorphic nature of CD90 in mice and the diverse research contexts in which this antibody is employed. Strain-Dependent VariationsThe most critical factor affecting OX-7 dosing is the specific CD90 allelic variant present in the mouse strain. Clone OX-7 exhibits differential reactivity based on whether mice express Thy1.1 or Thy1.2 alleles. The antibody reacts strongly with Thy1.1-expressing strains such as AKR/J, FVB, and PL mice, but does not bind to Thy1.2-expressing strains including CBA and BALB/c. This fundamental difference means that OX-7 can only be used effectively in Thy1.1 mouse strains, inherently limiting its application across different mouse models. The binding affinity itself varies between species and alleles, with the Fab′ fragment of OX-7 showing an affinity of 3 x 10⁸ M⁻¹ for mouse Thy1.1, which is tenfold lower than its 3 x 10⁹ M⁻¹ affinity for rat Thy1. This difference in binding strength likely necessitates adjustments in dosing when working with mouse versus rat models. Application-Specific DosingThe intended experimental application fundamentally shapes the dosing regimen employed. For flow cytometry applications, a standard approach uses 10 μL of the working dilution to label 1 x 10⁶ cells in 100 μL. However, this represents just one narrow application context, and dosing protocols are not standardized across different experimental purposes. The detection method being used also influences dosing decisions. Different analytical techniques—whether flow cytometry, fluorescence microscopy, or other immunological assays—require optimization of antibody concentration to achieve optimal signal-to-noise ratios and minimize background staining. Functional Study VariationsBeyond detection and analytical applications, functional studies using OX-7 demonstrate additional dosing complexity. The antibody has been used to promote neurite outgrowth in peripherin-stained sympathetic rat neurons, and has been reported to induce glomerular nephritis in Wistar rats. These diverse biological effects suggest that dosing must be carefully calibrated based on whether the goal is cell labeling, functional modulation, or disease induction. The lack of standardization across protocols means that exact dosing regimens are typically determined empirically for each specific combination of mouse strain, experimental model, and research objective. Researchers must consider the interplay between strain genotype, binding affinity, desired biological effect, and detection methodology when establishing appropriate dosing protocols for clone OX-7 in their particular mouse model system. References & Citations1. Jurisic G, Iolyeva M, Proulx ST, et. al. Exp Cell Res. 316: 2982–2992. 2010.
2. Ledbetter JA, Rouse RV, Micklem HS, et. al. J Exp Med. 152: 280–295. 1980. 3. Schrader JW, Battye F, Scollay R. Proc Natl Acad Sci. U S A 79: 4161–4165. 1982. 4. Phipps RP, Baecher C, Frelinger JG, et. al. Eur J Immunol. 20: 1723–1727. 1990. 5. Hu P, Leyton L, Hagood JS, Barker TH. Front Cell Dev Biol. Jun 6;10:928510. 2022. 6. Crook K, Hunt SV. Dev Immunol. 4(4):235-46. 1996. 7. Zhang J, Wang JH. Methods Mol Biol. 1842:217-228. 2018 8. Hermans MH, Opstelten D. J Histochem Cytochem. Dec;39(12):1627-34. 1991 Technical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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C851 | |
C876 | |
C875 | |
C873 | |
C874 |
