Anti-Mouse EpCAM (CD326) – Dylight® 550

Anti-Mouse EpCAM (CD326) – Dylight® 550

Product No.: C732

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Clone
G8.8
Target
CD326
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Alternate Names
EGP314
Isotype
Rat IgG2a κ
Applications
FC
,
IHC

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
State of Matter
Liquid
Storage and Handling
This DyLight® 550 conjugate is stable when stored at 2-8°C. Do not freeze.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2-8°C Wet Ice
Excitation Laser
Yellow Laser (563 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC
Additional Applications Reported In Literature ?
IHC,
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
G8.8 activity is directed against mouse EpCAM (CD326) and does not recognize human or rat EpCAM.
Background
Epithelial cell adhesion molecule (EpCAM; also known as CD326 or Tacstd1) is a 40 kDa type I transmembrane glycoprotein composed of an extracellular domain, single transmembrane domain, and the intracellular domain Ep1CD1. EpCAM functions in cell adhesion, signaling, differentiation, migration, proliferation, formation and maintenance of organ morphology, and morphogenic movements during gastrulation. Additionally, EpCAM is essential for cell junctions; the AxxxG motif in the transmembrane domain of EpCAM associates directly with claudin-7, an important tight junction protein. EpCAM also suppresses or enhances E-cadherin function depending on the context of the interaction. Mutant animal models have been developed in mouse (at least four global EpCAM knockout types and one conditional knockout), zebrafish, and Xenopus.

Dysregulation and/or mutations are associated with congenital tufting enteropathy (CTE), which causes lethal diarrhea in newborns, cholestatic liver diseases, and cancer1. EpCAM promotes the proliferation of tumors, is involved in tumorigenesis and metastasis, and EpCAM positive cells serve as cancer stem cells for various human cancers. Therapeutic approaches targeting EpCAM are under development to eliminate chemotherapeutic drug resistance in cancer stem cells by conjugating cancer stem cells targeting EpCAM aptamer with a chemotherapeutic drug. Additionally, EpCAM antibody sensitizes chemoresistant myeloid leukemia to innate immune cells, and EpCAM peptide-primed dendritic cell vaccinations exhibit anti-tumor immunity in hepatocellular carcinoma cells.

Monoclonal antibody G8.8 was raised against glycoconjugates isolated from the TE-71 mouse thymic epithelial cell line2. Splenic cell suspensions were fused with X63-Ag8.653 cells and the resulting hybridomas were screened on frozen Balb/c thymus.
Antigen Distribution
EpCAM is expressed in many epithelial tissues from very early embryos to adult animals and is a cell surface marker on various stem and progenitor cells. EpCAM is also an important carcinoma marker highly expressed on a variety of carcinomas, including epithelial tumors and acute myeloid leukemia. EpCAM is enriched in the basolateral membrane of mouse and human intestinal epithelium and is localized to tight junctions, adherens junctions, and the lateral membranes of epithelial cells lining the intestines.
Ligand/Receptor
LAIR-1 (CD305) and LAIR-2 (CD306)
NCBI Gene Bank ID
UniProt.org
Research Area
Cell Adhesion
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Immunology

References & Citations

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Flow Cytometry
IHC

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.