CHO Cell-Derived Recombinant Mouse EPO
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:R380. The detection sensitivity of this indirect ELISA for Mouse EPO is approximately 2 ng/well.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse EPO bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse EPO (at 40 ng/ml) is 0.2 - 0.8 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rat Anti-Mouse Erythropoietin (EPO) (Clone 148438) recognizes an epitope on Mouse EPO. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Erythropoietin or EPO is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. Also called hematopoietin or hemopoietin, it is produced by the kidney, and is the hormone that regulates red blood cell production.1 Erythropoietin has its primary effect on red blood cells by promoting red blood cell survival through protecting these cells from apoptosis. It also cooperates with various growth factors involved in the development of precursor red cells. It has a range of actions including vasoconstriction-dependent hypertension, stimulating angiogenesis, and inducing proliferation of smooth muscle fibers. Erythropoietin plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process.2
NCBI Gene Bank ID
References & Citations
1. Siren, AL. et al. (2001) Proc Natl Acad Sci USA 98: 4044
2. Haroon, ZA. et al. (2003) Am J Pathol 163: 993
Products are for research use only. Not for use in diagnostic or therapeutic procedures.