Anti-Human/Mouse Integrin β7 – Biotin

TK1 Cells

This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

The antibody was purified by affinity chromatography, and biotinylated under optimal conditions.

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Next Day 2-8°C

Additional reported applications (for the relevant formats) include:
B The functional grade GOLD (Part No.: I-1141) should be used to block beta7 integrin mediated cell adhesion for In vitro and In vivo studies.
Western Blotting
Immunoprecipitation

Clone FIB504 recognizes an epitope on human/mouse integrin β7.

Integrin β7 antibody, clone FIB504, recognizes integrin β7, a 130 kDa membrane glycoprotein of the Ig superfamily. Integrin β7 forms heterodimers with both the α4 (CD49d) and aE (CD103) integrins. α4β7 integrin is expressed on subsets of peripheral lymphocytes, thymocytes, and bone marrow progenitors^1,2. It mediates adhesion to mucosal endothelial cells, promoting leukocyte transendothelial migration through interactions with mucosal addressin cell adhesion molecule-1 (MAdCAM-1)^3,4. The αEβ7 integrin is expressed on mucosal T cells, including intraepithelial T lymphocytes (IELs) and lamina propria T cells^5,6, subsets of dendritic cells⁷, and regulatory T cells (Tregs)8. It facilitates retention in the gut epithelial layer via interactions with E-cadherin⁹. Anti-β7 antibodies block both the homing to and retention in the gut of pathogenic T cells¹⁰ and are currently under evaluation in a phase 3 clinical trial to treat inflammatory bowel disease¹¹.

Integrin α4β7 is expressed on subsets of peripheral lymphocytes, thymocytes, and bone marrow progenitors. The αEβ7 integrin is expressed on mucosal T cells, including intraepithelial T lymphocytes (IELs) and lamina propria T cells, subsets of dendritic cells, and regulatory T cells (Tregs)⁸.

CD49d/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/β7: E-cadherin

Lymphocyte adhesion, hematopoietic progenitor cells migration

1. Andrew DP, et al. (1996) Eur J Immunol. 26(4):897-905
2. Murakami JL, et al. (2016) Stem Cells Dev. 25(1):18-26
3. Hu MC, et al. (1992) Proc Natl Acad Sci USA. 89(17):8254-8
4. Hamann A, et al. (1994) J Immunol. 152(7):3282-93
5. Hadley GA Higgins JM. (2014) Adv Exp Med Biol. 819:97–110
6. Farstad IN., et al. (1996) Immunology. 89:227–37
7. Jaensson E., et al. (2008) J Exp Med. 205:2139–49
8. Allez M, et al. (2002) Gastroenterology. 123(5):1516-26
9. Schön MP, et al. (1999) J Immunol. 162(11):6641-9
10. Stefanich EG, et al. (2011) Br J Pharmacol. 162(8):1855-1870
11. Smids C, et al. (2017) J Crohns Colitis. 11(4):500-508