≥ 2.0 mg/ml
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
Functional Assay: In vitro activity of TIF-3C5 - inhibits IFNα-induced (IFNα-A, -1, -4, -5, -11 and 13) Stat1 phosphorylation in vitro. [TIF-1D6 does not block in vitro functional activity]. - blocks IFNα (IFNα-A, -1, -4, -5, -11 and 13) induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines in a dose dependent manner. - neutralizes IFNα-induced antiviral activity in vitro following infection of L929 cells with VSV. Functional activity of both recombinant (IFNα-A, -1, -4, -5, -11 and 13) and natural IFNα can be blocked with no neutralization of IFNgor IFNb. In vivo activity of TIF-3C5 - TIF-3C5 circulates with a half-life of 4 days - blockade of IFNa by TIF-3C5 increases the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of Irf7-/- mice.
Armenian Hamster Anti-Mouse IFNα (Clone TIF-3C5) binds specifically to murine IFNa species (IFN-αA, -1, -4, -5, -11. and -13) and does not bind murine IFNg or IFNb . TIF-3C5 binds both recombinant and natural forms of IFNα. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern.
References & Citations
1. Oldstone, Michael B. A. et al. (2017) Proc Natl Acad Sci U S A. 114(14): 3708–3713. PubMed
Products are for research use only. Not for use in diagnostic or therapeutic procedures.