Anti-Mouse MAdCAM-1 (MECA-367) – Purified in vivo PLATINUMTM Functional Grade
Anti-Mouse MAdCAM-1 (MECA-367) – Purified in vivo PLATINUMTM Functional Grade
Product No.: M1408
Clone MECA-367 Target MADCAM-1 Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names Mucosal addressin cell adhesion molecule-1 Isotype Rat IgG2a κ Applications FA , FC , IF , IHC , IP , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen Endothelial Cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level ≤ 0.5 EU/mg as determined by the LAL method Purity ≥95% by SDS Page ⋅ ≥98% monomer by analytical SEC Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. State of Matter Liquid Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Regulatory Status Research Use Only Country of Origin USA Shipping 2 - 8°C Wet Ice Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity MECA-367 activity is directed against mouse MAdCAM-1. Background MAdCAM-1 is a cell adhesion leukocyte receptor expressed by mucosal venules that helps direct lymphocyte traffic into mucosal tissues and regulates the passage and retention of leukocytes1, 2. MAdCAM-1 binds integrin receptor α4β7 and L-selectin2, 3, 4. Two alternatively spliced isoforms of MAdCAM-1 exist5, both of which are capable of binding α4β72.
MECA-367 was generated by immunizing Wister rats with endothelial cells isolated from BALB/c mesenteric and peripheral lymph nodes6. Immunohistological screening of hybridomas yielded two mAbs, MECA-367 and MECA-89, that stain high endothelial venules (HEVs) in mucosal lymphoid organs and Peyer’s patches, but not peripheral lymph nodes (axillary, brachial, popliteal, and inguinal). Immunofluorescence staining of high endothelial cells shows that both MECA-367 and MECA-89 react with antigens on the cell surface. The epitopes for MECA-367 and MECA-89 are distinct. MECA-367 recognizes the N-terminal immunoglobulin domain of MAdCAM-l, and MECA-89 recognizes the second immunoglobulin domain4, 5. MECA-367 inhibits the binding of normal and neoplastic lymphocytes to HEVs in mucosa-associated lymphoid organs and Peyer’s patches6. In contrast, MECA-89 reacts with the same vessels, binds to isolated MECA-367 antigen, but has no effect on lymphocyte binding. In vivo, MECA-367 blocks homing of normal lymphocytes to mucosa-associated lymphoid tissues6. MECA-367 also inhibits α4β7 binding3 and blocks sticking and rolling of preactivated lymph node cells4. Antigen Distribution MAdCAM-1 is a cell surface glycoprotein selectively expressed on high endothelial venules of mucosal lymphoid organs and Peyer’s patches as well as lamina propria venules. Ligand/Receptor Integrin a4ß7, CD62L PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone MECA-367 is an anti-mouse MAdCAM-1 monoclonal antibody commonly used in in vivo applications in mice to neutralize MAdCAM-1, thereby preventing lymphocytes, particularly those expressing α4β7 integrin (LPAM-1) and CD62L, from tethering, rolling, and homing to gut-associated lymphoid tissues such as Peyer's patches and mesenteric lymph nodes. This antibody is utilized to study and modulate the homing of lymphocytes to these tissues, which is crucial for understanding inflammatory conditions like Crohn's disease. Key in vivo applications include:
Commonly used antibodies or proteins in the literature with MECA-367 (an anti-MAdCAM-1 monoclonal antibody) include isotype controls (such as rat IgG2a, e.g., R35–95), other adhesion molecules like VCAM-1, and proteins or antibodies associated with lymphocyte homing, including PF-00547659 (an anti-human MAdCAM-1 antibody), as well as markers such as LPAM-1 (α4β7 integrin) and CD62L (L-selectin). Key antibodies or proteins used with MECA-367:
These combinations enable researchers to dissect the role of MAdCAM-1 in immune cell trafficking, validate antibody specificity, and control for non-specific effects in both in vivo and in vitro experiments. Key Findings from MECA-367 Scientific Literature CitationsMECA-367 is a well-characterized rat monoclonal antibody targeting murine mucosal addressin cell adhesion molecule-1 (MAdCAM-1). Its citations in the literature have contributed significantly to understanding the physiological and pathological roles of MAdCAM-1, particularly in lymphocyte trafficking and inflammatory disease models. Functional Role and Mechanism
Effects in Experimental Models
Scientific and Translational Significance
Summary Table: Key Functional Outcomes of MECA-367
ConclusionMECA-367 is a critical tool for studying MAdCAM-1 biology, demonstrating that blockade of the MAdCAM-1/α4β7 axis prevents lymphocyte homing to mucosal tissues, reduces inflammation in models of IBD, and modulates autoimmune responses. Its use has advanced both basic research and the development of therapeutic strategies targeting this pathway in human disease. Dosing regimens for clone MECA-367 in mouse models most often involve single intravenous (IV) injections at increasing doses, typically ranging from 0.1, 0.3, 1, to 3 mg·kg⁻¹. The optimal effect on target cell populations (e.g., an increase in circulating β7⁺ or LPAM⁺ memory T-cells) is generally observed around 1 mg·kg⁻¹, with higher doses sometimes used for dose–response evaluation. Essential details and context:
Comparison across mouse models:
Additional notes:
In summary, dosing regimens of clone MECA-367 are consistent across mouse models, characterized by single IV injection of escalating doses (0.1–3 mg·kg⁻¹), mostly evaluated at 1 mg·kg⁻¹, with immune readouts 3–7 days post-dose. Published data do not indicate substantial variation in regimen by mouse strain or disease context, though downstream effects may differ due to biological variables between models. References & Citations1. https://www.uniprot.org/uniprotkb/Q61826/entry
2. Schiffer SG, Day E, Latanision SM, et al. Biochem Biophys Res Commun. 216(1):170-176. 1995. 3. Berlin C, Berg EL, Briskin MJ, et al. Cell. 74(1):185-195. 1993. 4. Bargatze RF, Jutila MA, Butcher EC. Immunity. 3(1):99-108. 1995. 5. Briskin MJ, McEvoy LM, Butcher EC. Nature. 363(6428):461-464. 1993. 6. Streeter PR, Berg EL, Rouse BT, et al. Nature. 331(6151):41-46. 1988. 7. Berlin C, Bargatze RF, Campbell JJ, et al. Cell. 80(3):413-422. 1995. 8. Nakache M, Berg EL, Streeter PR, et al. Nature. 337(6203):179-181. 1989. Technical ProtocolsCertificate of Analysis |
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