Anti-Mouse MAdCAM-1 (MECA-89) – FITC

Anti-Mouse MAdCAM-1 (MECA-89) – FITC

Product No.: M1425

[product_table name="All Top" skus="M1400"]

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Clone
MECA-89
Target
MADCAM-1
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Alternate Names
Mucosal addressin cell adhesion molecule-1
Isotype
Rat IgG2a κ
Applications
FC

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Mouse mesenteric and peripheral lymph node cells
Product Concentration
0.2 mg/ml
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
State of Matter
Liquid
Storage and Handling
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 - 8°C Wet Ice
Excitation Laser
Blue Laser (490 nm)
Additional Applications Reported In Literature ?
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
MECA-89 activity is directed against mouse MAdCAM-1.
Antigen Distribution
MAdCAM-1 is a cell surface glycoprotein selectively expressed on high endothelial venules of mucosal lymphoid organs and Peyer’s patches as well as lamina propria venules.
Background
MAdCAM-1 is a cell adhesion leukocyte receptor expressed by mucosal venules that helps direct lymphocyte traffic into mucosal tissues and regulates the passage and retention of leukocytes1, 2. MAdCAM-1 binds integrin receptor α4β7 and L-selectin2, 3, 4. Two alternatively spliced isoforms of MAdCAM-1 exist5, both of which are capable of binding α4β72.

MECA-89 was generated by immunizing Wistar rats with endothelial cells isolated from BALB/c mesenteric and peripheral lymph nodes 6. Immunohistological screening of hybridomas yielded two mAbs, MECA-367 and MECA-89, that stain high endothelial venules (HEVs) in mucosal lymphoid organs and Peyer’s patches, but not peripheral lymph nodes (axillary, brachial, popliteal, and inguinal). Immunofluorescence staining of high endothelial cells shows that both MECA-367 and MECA-89 react with antigens on the cell surface. The epitopes for MECA-367 and MECA-89 are distinct. MECA-367 recognizes the N-terminal immunoglobulin domain of MAdCAM-l, and MECA-89 recognizes the second immunoglobulin domain 4,5.

MECA-89 reacts with the same vessels as MECA-367 and binds to isolated MECA-367 antigen; however, unlike MECA-367 it has no effect on lymphocyte binding 6. Additionally, MECA-89 has no effect on MAdCAM-1 binding to α4β7 in vitro 7, but L-selectin dependent adhesion is lost in the presence of MECA-89 4. Additionally, MECA-89 has no significant effect on activated cells, but all interactions are inhibited following subsequent injection of anti-α4 Fab fragments 4. In vivo, MECA-89 inhibits L-selectin-dependent rolling but not direct α4β7-dependent attachment of Mn2+ activated lymphocytes.

Antigen Details

Ligand/Receptor
Integrin a4ß7, CD62L
NCBI Gene Bank ID
UniProt.org
Research Area
Cell Adhesion
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Cell Biology
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Immunology

References & Citations

1. https://www.uniprot.org/uniprotkb/Q61826/entry
2. Schiffer SG, Day E, Latanision SM, et al. Biochem Biophys Res Commun. 216(1):170-176. 1995.
3. Berlin C, Berg EL, Briskin MJ, et al. Cell. 74(1):185-195. 1993.
4. Bargatze RF, Jutila MA, Butcher EC. Immunity. 3(1):99-108. 1995.
5. Briskin MJ, McEvoy LM, Butcher EC. Nature. 363(6428):461-464. 1993.
6. Streeter PR, Berg EL, Rouse BT, et al. Nature. 331(6151):41-46. 1988.
7. Nakache M, Berg EL, Streeter PR, et al. Nature. 337(6203):179-181. 1989.
Flow Cytometry
Products are for research use only. Not for use in diagnostic or therapeutic procedures.