Anti-Mouse MHC Class I (H-2Kb) (Clone AF6-88.5) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse MHC Class I (H-2Kb) (Clone AF6-88.5) – Purified in vivo GOLD™ Functional Grade
Product No.: H1686
Clone AF6-88.5 Target MHC Class I Formats AvailableView All Product Type Monoclonal Antibody Alternate Names H-2Kb Isotype Mouse IgG2a k Applications FA , FC , IHC FF , in vivo , IP |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen Mouse C57BL Splenocytes Product Concentration ≥ 5.0 mg/ml Endotoxin Level <1.0 EU/µg as determined by the LAL method Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2893717 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for clone AF6-88.5 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IP IHC FF FA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone AF6-88.5, Anti-Mouse H-2Kb, recognizes an epitope on mouse MHC class I H-2K haplotype b. AF6-88.5 does not cross-react with other H-2K haplotypes, including d, f, j, k, p, q, r, s, u, v. Background H-2Kb antibody, clone A4C8.1-Do9, recognizes the major histocompatibility complex (MHC) class I H-2K haplotype b (H-2Kb). MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2Kb plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I to augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11. Antigen Distribution The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen and is ubiquitously expressed on nucleated cells from mice of the H-2Kb haplotype, including C57BL/6. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.
Ligand/Receptor CD3/TCR, CD8 Function Antigen presentation PubMed NCBI Gene Bank ID Research Area Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. The AF6-88.5 clone is a mouse monoclonal antibody that specifically recognizes the H-2K^b MHC class I alloantigen on mouse cells, and it is widely used in in vivo mouse studies for several immunological applications. Key uses in in vivo mouse studies
Usage specifics
In summary: Commonly used antibodies or proteins in combination with AF6-88.5 (anti-mouse MHC class I H-2K^b) in the literature include those targeting major cell surface markers and other MHC molecules, especially in immunological assays and mouse model studies. Key antibodies/proteins frequently used alongside AF6-88.5
Typical applicationsThese combinations are common in:
where multiparametric analysis of immune cell populations and their activation status is required. For optimal experimental design, researchers also use:
In summary: Antibodies against CD8, CD4, other MHC-I proteins (H-2D^b, H-2L^d), CD3/TCR, and lineage markers are most commonly used alongside AF6-88.5 in immunological studies and mouse model research. Clone AF6-88.5 is a well-characterized monoclonal antibody that specifically recognizes the mouse MHC class I H-2K^b alloantigen and is widely used in immunology research for detecting and analyzing H-2K^b expression. Key findings from scientific literature and product citations include:
Notable Scientific Citations:
In summary, clone AF6-88.5 is a standard, highly specific antibody for detecting murine H-2K^b in immunological contexts, with well-documented performance in cell staining, antigen presentation studies, and Fc receptor analysis in mice of the H-2K^b haplotype. Dosing regimens for clone AF6-88.5 (an anti-mouse MHC class I H-2K^b monoclonal antibody) vary primarily by application and are generally not highly dependent on specific mouse models, but rather on the experimental use and strain's H-2K^b expression. The most common dosing information available concerns in vitro and ex vivo assay applications, with limited published data on in vivo dosing in mouse models. Essential context and supporting details:
Additional relevant information:
In summary, dosing regimens of clone AF6-88.5 are driven by application type (mostly in vitro/ex vivo assays) and H-2K^b expression, not by mouse disease model, since its use depends on the presence of the correct MHC haplotype, and there is no standard in vivo dosing protocol available for this clone. Investigators must optimize working concentrations for each assay and mouse strain, and consult up-to-date datasheets for lot-specific instructions. References & Citations1. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25 2. Wieczorek M, et al. (2017) Front Immunol. 8:292 3. Jones EY. (1997) Curr Opin Immunol. 9(1):75-9 4. Matsumura M, et al. (1992) Science. 257:927–34.10.1126/science.1323878 5. Bouvier M & Wiley DC. (1994) Science. 265:398–402.10.1126/science.8023162 6. Zacharias M & Springer S. (2004) Biophys J. 87:2203–14.10.1529/biophysj.104.044743 7. Cruz FM, et al (2017) Annu Rev Immunol. 35:149-176 8. Artyomov MN, et al (2010) Proc Natl Acad Sci USA. 107(39):16916-16921 9. Orr MT & Lanier LL. (2010) Cell. 142(6):847-856 10. Raulet DH. (1994) Adv Immunol. 55:381-421 11. Salcedo M & Ljunggren HG. (1996) Chem Immunol. 64:44-58 Technical ProtocolsCertificate of Analysis |
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