Anti-Mouse MHC class II (I-A) (Clone Y-3P) – Purified in vivo GOLD™ Functional Grade

Anti-Mouse MHC class II (I-A) (Clone Y-3P) – Purified in vivo GOLD™ Functional Grade

Product No.: H470

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Clone
Y-3P
Target
MHC class II (I-A)
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Isotype
Mouse IgG2a k
Applications
B
,
FC

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Recommended Dilution Buffer
Immunogen
BALB/c x C57BL/6 F1 mouse spleen cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Liquid
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Additional Applications Reported In Literature ?
B,
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Y-3P activity is directed against mouse MHC class II (I-A) glycoprotein antigens, including the haplotypes I-Ab, I-Af, I-Ap, I-Aq, I-Ar, I-As, I-Au, I-Av, and weakly I-Ak. Y-3P also reacts with the equivalent complexes in rats.
Background
H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response1. Class II molecules, also known as Ia antigens, regulate recognition of foreign antigens on the surfaces of antigen presenting cells and play a major role in the mixed lymphocyte response2. Mice have two class II isotypes, I-A and I-E, each of which is a glycoprotein composed of an ⍺ and β subunit. The N-terminal α1 and β1 domains of the MHC class II isotype form the antigen- binding groove, which binds 13-25 aa peptides derived from exogenous antigens3.

On APCs, MHC class II molecules play a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling4. Additionally, MHC class II expressed on activated T cells are capable of antigen presentation5 and can transduce signals into T cells, enhancing T cell proliferation and activity6.

Y-3P was generated by repeatedly immunizing primed mice with activated T cells over the course of a year7. Y-3P reacts with I-A subregion-controlled A ⍺: A β complexes of all mouse strains except the responder strain H-2d. Y-3P is commonly used for in vivo blockade of TCR stimulation8,9 and MHC class II blocking10,11,12,13,14,15.

Antigen Distribution
MHC class II molecules are constitutively expressed on professional antigen-presenting cells (APCs), including macrophages, monocytes, dendritic cells (DCs), and B cells, and are induced on T cells upon activation.
Ligand/Receptor
CD3/TCR, CD4
NCBI Gene Bank ID
UniProt.org
Research Area
Immunology
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Innate Immunity

References & Citations

1. Yoshida R. Adv Immunol. 124:207-247. 2014.
2. Spencer JS, Kubo RT. J Exp Med. 169(3):625-640. 1989.
3. Wieczorek M, Abualrous ET, Sticht J, et al. Front Immunol. 8:292. 2017.
4. Artyomov MN, Lis M, Devadas S, et al. Proc Natl Acad Sci USA. 107(39):16916-16921. 2010.
5. Barnaba V, Watts C, de Boer M, et al. Eur J Immunol. 24(1):71-75. 1994.
6. Di Rosa F, D'Oro U, Ruggiero G, et al. Hum Immunol. 38(4):251-260. 1993.
7. Janeway CA Jr, Conrad PJ, Lerner EA, et al. J Immunol. 132(2):662-667. 1984.
8. Feng Y, van der Veeken J, Shugay M, et al. Nature. 528(7580):132-136. 2015.
9. Campisi L, Barbet G, Ding Y, et al. Nat Immunol. 17(9):1084-1092. 2016.
10. Stefanová I, Dorfman JR, Germain RN. Nature. 420(6914):429-434. 2002.
11. Andersson J, Stefanova I, Stephens GL, et al. Int Immunol. 19(4):557-566. 2007.
12. Younes SA, Punkosdy G, Caucheteux S, et al. PLoS Biol. 9(10):e1001171. 2011.
13. Guo L, Huang Y, Chen X, et al. Nat Immunol. 16(10):1051-1059. 2015.
14. Kawabe T, Yi J, Kawajiri A, et al. Nat Commun. 11(1):3366. 2020.
15. Kruse B, Buzzai AC, Shridhar N, et al. Nature. 618(7967):1033-1040. 2023.
16. Wei J, Loke P, Zang X, et al. J Exp Med. 208(8):1683-1694. 2011.
17. Alspach E, Lussier DM, Miceli AP, et al. Nature. 574(7780):696-701. 2019.
18. Hos BJ, Tondini E, Camps MGM, et al. Cell Rep. 41(2):111485. 2022.
B
Flow Cytometry

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.