Anti-Mouse MHC Class II (I-A) [Clone Y-3P] – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse MHC Class II (I-A) [Clone Y-3P] – Purified in vivo PLATINUM™ Functional Grade
Product No.: H471
Clone Y-3P Target MHC class II (I-A) Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Isotype Mouse IgG2a k Applications B , FC |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Recommended Dilution Buffer Immunogen BALB/c x C57BL/6 F1 mouse spleen cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. State of Matter Liquid Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM<sup>TM</sup> antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Additional Applications Reported In Literature ? B, FC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Y-3P activity is directed against mouse MHC class II (I-A) glycoprotein antigens,
including the haplotypes I-Ab, I-Af, I-Ap, I-Aq, I-Ar, I-As, I-Au, I-Av, and weakly I-Ak. Y-3P
also reacts with the equivalent complexes in rats. Background H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of
antigens divided into class I and II proteins that function in immune response1. Class II
molecules, also known as Ia antigens, regulate recognition of foreign antigens on the surfaces of
antigen presenting cells and play a major role in the mixed lymphocyte response2. Mice have
two class II isotypes, I-A and I-E, each of which is a glycoprotein composed of an ⍺ and β
subunit. The N-terminal α1 and β1 domains of the MHC class II isotype form the antigen-
binding groove, which binds 13-25 aa peptides derived from exogenous antigens3. On APCs, MHC class II molecules play a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling4. Additionally, MHC class II expressed on activated T cells are capable of antigen presentation5 and can transduce signals into T cells, enhancing T cell proliferation and activity6. Y-3P was generated by repeatedly immunizing primed mice with activated T cells over the course of a year7. Y-3P reacts with I-A subregion-controlled A ⍺: A β complexes of all mouse strains except the responder strain H-2d. Y-3P is commonly used for in vivo blockade of TCR stimulation8,9 and MHC class II blocking10,11,12,13,14,15. Antigen Distribution MHC class II molecules are constitutively expressed on professional
antigen-presenting cells (APCs), including macrophages, monocytes, dendritic cells (DCs), and
B cells, and are induced on T cells upon activation. Ligand/Receptor CD3/TCR, CD4 NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. The Y-3P clone is primarily used in in vivo mouse studies for immunological blockade experiments and MHC class II functional studies. This monoclonal antibody serves as a powerful research tool for manipulating immune responses in living mice. Primary Applications in In Vivo StudiesTCR Stimulation Blockade: Y-3P is commonly used for in vivo blockade of T cell receptor (TCR) stimulation. By binding to MHC class II molecules on antigen-presenting cells, the antibody prevents proper antigen presentation to CD4+ T cells, effectively blocking T cell activation. This application is valuable for studying T cell-dependent immune responses and investigating the role of specific T cell populations in various disease models. MHC Class II Blocking: The antibody is extensively used for MHC class II blocking in various experimental contexts. This blocking capability allows researchers to study the functional importance of MHC class II-mediated antigen presentation in immune responses, transplant rejection, autoimmune diseases, and other immunological processes. Mechanism of ActionY-3P works by binding to I-A subregion-controlled A?:A? complexes of MHC class II molecules. The antibody reacts with multiple mouse MHC class II haplotypes including I-Ab, I-Af, I-Ap, I-Aq, I-Ar, I-As, I-Au, I-Av, and weakly with I-Ak, but notably does not react with the H-2d haplotype. This broad reactivity makes it useful across different mouse strains, though researchers must consider the H-2d limitation when designing experiments. Research ApplicationsThe Y-3P antibody is particularly valuable in transplantation studies where researchers need to block immune recognition and rejection. It's also used in autoimmune disease models where MHC class II-restricted T cell responses play a crucial role. Additionally, the antibody serves as an important tool in stem cell research, where understanding immune rejection mechanisms is critical for therapeutic applications. The antibody's ability to inhibit I-A-restricted T cell responses makes it an essential reagent for dissecting the contributions of different immune pathways in complex in vivo systems, allowing researchers to determine whether observed effects are dependent on MHC class II-mediated antigen presentation. Sterile packaged Clone Y-3P should be stored at 2–8°C (refrigerator temperature) for up to one month if kept sterile as received. For long-term storage, aliquot aseptically (without dilution) and store at ? -70°C. It is important to avoid repeated freeze-thaw cycles, as this can reduce antibody integrity.
This storage practice ensures the antibody's viability and functional quality for downstream applications. Always consult the product's batch-specific datasheet for any protocol updates. The Y-3P antibody, which targets mouse MHC class II (I-A) molecules, is commonly used alongside several other key proteins and antibodies in immunological research, particularly for studies involving antigen presentation and T cell activation. T Cell Receptor ComponentsY-3P frequently appears in studies examining T cell receptor signaling pathways. The CD3/TCR complex serves as a primary ligand/receptor system that Y-3P interacts with, as MHC class II molecules present antigens to T cell receptors. This interaction is fundamental to adaptive immune responses, making CD3 and various TCR subunits common research targets when using Y-3P for blocking experiments. CD4 Co-receptorThe CD4 molecule is another critical component frequently studied with Y-3P. CD4 T cells directly bind to MHC class II molecules, which helps augment TCR signaling. Since Y-3P blocks MHC class II function, researchers often examine CD4 T cell responses and activation states when using this antibody for in vivo blockade studies. Antigen-Presenting Cell MarkersGiven that MHC class II molecules are constitutively expressed on professional antigen-presenting cells, Y-3P is commonly used with markers for various APC populations. These include:
Activation MarkersSince MHC class II molecules are induced on T cells upon activation, Y-3P studies frequently incorporate T cell activation markers to assess the functional consequences of MHC class II blockade. These markers help researchers understand how blocking antigen presentation affects overall immune cell activation states. Secondary Detection SystemsIn experimental applications, Y-3P is often used with secondary antibodies for detection purposes, particularly in techniques like flow cytometry, immunofluorescence, and western blotting. The biotin-conjugated version of Y-3P specifically requires streptavidin-based detection systems. The versatility of Y-3P in blocking TCR stimulation and MHC class II function makes it a valuable tool for dissecting complex immune interactions, explaining why it's frequently paired with these diverse immunological markers and detection systems. Clone Y-3P is a monoclonal antibody that targets Mouse MHC Class II (I-A) molecules. It is primarily used in immunological research for in vivo blockade of T cell receptor (TCR) stimulation and MHC Class II blocking. Here are the key findings from citations involving clone Y-3P:
In summary, clone Y-3P is a valuable tool in immunological research, particularly for studying the function of MHC Class II molecules in antigen presentation and T cell activation. References & Citations1. Yoshida R. Adv Immunol. 124:207-247. 2014. 2. Spencer JS, Kubo RT. J Exp Med. 169(3):625-640. 1989. 3. Wieczorek M, Abualrous ET, Sticht J, et al. Front Immunol. 8:292. 2017. 4. Artyomov MN, Lis M, Devadas S, et al. Proc Natl Acad Sci USA. 107(39):16916-16921. 2010. 5. Barnaba V, Watts C, de Boer M, et al. Eur J Immunol. 24(1):71-75. 1994. 6. Di Rosa F, D'Oro U, Ruggiero G, et al. Hum Immunol. 38(4):251-260. 1993. 7. Janeway CA Jr, Conrad PJ, Lerner EA, et al. J Immunol. 132(2):662-667. 1984. 8. Feng Y, van der Veeken J, Shugay M, et al. Nature. 528(7580):132-136. 2015. 9. Campisi L, Barbet G, Ding Y, et al. Nat Immunol. 17(9):1084-1092. 2016. 10. Stefanová I, Dorfman JR, Germain RN. Nature. 420(6914):429-434. 2002. 11. Andersson J, Stefanova I, Stephens GL, et al. Int Immunol. 19(4):557-566. 2007. 12. Younes SA, Punkosdy G, Caucheteux S, et al. PLoS Biol. 9(10):e1001171. 2011. 13. Guo L, Huang Y, Chen X, et al. Nat Immunol. 16(10):1051-1059. 2015. 14. Kawabe T, Yi J, Kawajiri A, et al. Nat Commun. 11(1):3366. 2020. 15. Kruse B, Buzzai AC, Shridhar N, et al. Nature. 618(7967):1033-1040. 2023. 16. Wei J, Loke P, Zang X, et al. J Exp Med. 208(8):1683-1694. 2011. 17. Alspach E, Lussier DM, Miceli AP, et al. Nature. 574(7780):696-701. 2019. 18. Hos BJ, Tondini E, Camps MGM, et al. Cell Rep. 41(2):111485. 2022. Technical ProtocolsB  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
