Anti-Mouse TCR β chain – Purified in vivo PLATINUM™ Functional Grade

The clone H57-597, a hamster monoclonal antibody, is widely used in mice for several in vivo applications due to its specificity for the TCR beta chain of mouse T cells. Common applications include:

• In Vivo Depletion: H57-597 can effectively deplete TCR α/β-bearing T cells, which is often used to study T cell function and immune responses in vivo.
• Immune Regulation: It is used to induce long-term allograft survival by reducing T-cell responses to allo-antigens and increasing regulatory T cell frequency in lymphoid organs.
• Immune Modulation: The antibody can activate T cells when immobilized, and it modulates immune responses by inducing apoptosis in conventional T cells while sparing regulatory T cells to some extent.

Overall, H57-597 is a versatile tool for manipulating T cell populations in mouse models of immune function and disease.

Based on the literature, several antibodies and proteins are commonly used in combination with H57-597 for various immunological assays and T cell studies:

CD3 is frequently paired with H57-597 as it represents a core component of the TCR complex and serves as a classic T cell marker. Anti-CD3 antibodies, particularly clone 145-2C11, are often combined with H57-597 to define total T cell populations or for functional assays such as T cell activation.

CD4 is another commonly used marker alongside H57-597, as it identifies helper T cells (Th cells). Since H57-597 recognizes the beta chain of the αβ TCR expressed by the majority of peripheral T cells, combining it with CD4 allows researchers to specifically identify and study CD4+ T helper cell populations.

CD8 is used to identify cytotoxic T cells and is relevant when studying T cell subsets, as most T cells expressing the αβ TCR are either CD4+ or CD8+ single positive cells. The combination of H57-597 with CD8 markers enables the characterization of cytotoxic T cell populations.

These antibody combinations allow researchers to comprehensively phenotype T cell populations, distinguishing between different T cell subsets while confirming αβ TCR expression. The pairing of H57-597 with these markers is particularly valuable in flow cytometry panels where multiple parameters need to be assessed simultaneously to understand T cell composition and functional states in various experimental models.

Clone H57-597 is a monoclonal antibody highly specific for the beta chain of the mouse α/β T cell receptor (TCR) and is one of the most widely cited tools in immunology for mouse T cell phenotyping, activation, and functional studies. Key findings from scientific literature citing clone H57-597 include:

• Specificity and Application
  H57-597 binds specifically to the β-chain of the murine α/β TCR and does not react with γ/δ TCR. It is primarily used as a phenotypic marker to identify and isolate α/β T cells by flow cytometry and immunohistochemistry.

• Functional Impact and T Cell Activation
  When used in immobilized form, H57-597 can activate T cells via cross-linking the TCR complex, recapitulating physiological TCR-mediated signaling. This can induce proliferation, cytokine production, differentiation, or apoptosis, depending on the context.

• In Vivo Depletion
  Functionalized H57-597 antibody has been used for in vivo depletion of α/β T cells in mouse models to study T cell contributions to immunity, autoimmunity, and transplantation.

• Allograft and Immunomodulation Studies
  Transient treatment with H57-597 can exert long-term effects in suppressing T cell responses—notably, it has been reported to promote long-term cardiac allograft survival in mice by reducing alloreactive T cell numbers.

• Structural and Epitope Analysis
  Structural studies demonstrate that H57-597 recognizes a conserved epitope on the constant region of the TCR β-chain, which enables its broad application across mouse TCR analysis, including engineered TCRs.

• No Cross-Reactivity
  The antibody does not cross-react with γ/δ TCR-bearing T cells, ensuring specificity in studies involving diverse T cell subsets.

• Use in Genetic and Functional Assays
  H57-597 is used in experiments to explore TCR signaling, immune cell phenotyping, genetic manipulation of TCRs, and as part of multimarker panels in immunological studies.

Summary Table: Key Uses and Findings for Clone H57-597

These findings establish H57-597 as a benchmark reagent for studying mouse T cell biology, immune responses, and experimental immunotherapy.

Dosing regimens of the monoclonal antibody clone H57-597 vary across mouse models primarily in the dose amount and frequency, which are tailored to the experimental objective (such as T cell depletion, immune tolerance, or modulation), the mouse strain, and the disease or transplantation context.

Key details based on published studies and manufacturer data:

• For C57BL/6 (B6) mice, a single intraperitoneal injection of 1 mg/kg H57-597 is commonly used to induce T-cell depletion and modulate immune responses, such as increasing regulatory T cells (Treg) and enabling allograft survival.
• In some studies, multiple lower doses (5–50 μg) are administered over several days, particularly for protocols aiming at immune modulation without full depletion.
• When used in genetically modified mice, such as Bcl-2 transgenic (Tg) mice (which are resistant to apoptosis), the same 1 mg/kg dose fails to deplete conventional T cells or enrich Tregs, showing that dose efficacy can depend on genetic background.
• The experimental context matters: For transplant tolerance or T-cell depletion, higher and/or single doses are favored, while immune modulation protocols may employ lower repeated doses.
• Recovery of T-cell numbers after treatment is gradual, occurring over 40–100 days post-injection in standard B6 mice.

Typical use cases:

• Phenotypic marker: Lower doses may be used for flow cytometry or in vitro assays as a marker for TCR α/β+ cells, but these are unrelated to in vivo depletion.
• Activation or cross-linking: Immobilized antibody may be used to activate T cells in vitro, distinct from in vivo depletion protocols.

Summary table of typical dosing regimens for clone H57-597:

In summary, the regimen for H57-597 must be selected based on mouse strain, experimental aim, and desired immunological effect. Most published protocols in wild-type B6 mice use 1 mg/kg per injection, while exploratory or modulatory uses may employ repeated lower doses. Genetically altered models may show resistance to depletion at standard doses.