Anti-Mouse TCR β chain – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.T662
Clone
H57-597
Protein
TCR beta
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster
IgG
Applications
CODEX®
,
Costim
,
Depletion
,
FC
,
IHC FF
,
IHC FFPE
,
IP
Prod No.
Size
Price
Avail.
Qty
Add to cart
T662-1.0 mg
1.0 mg
$95.00
In stock
Max:
Min: 1
Step: 1
T662-5.0 mg
5.0 mg
$298.00
In stock
Max:
Min: 1
Step: 1
T662-25 mg
25 mg
$800.00
In stock
Max:
Min: 1
Step: 1
T662-50 mg
50 mg
$1,325.00
In stock
Max:
Min: 1
Step: 1
T662-100 mg
100 mg
$1,998.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this TCR β chain (clone H57-597) antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
Costim
Depletion
IP
IHC FF
IHC FFPE
Additional Reported Applications For Relevant Conjugates ?
CODEX®
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells.
Antigen Distribution
TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells.
Background
TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells1. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond2. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling3,4. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival5,6.

Antigen Details

Protein
Ligand/Receptor
Peptide bound-MHC class I and II
Function
Antigen recognition, T cell activation
PubMed
NCBI Gene Bank ID

References & Citations

1. Krijgsman D, et al. (2018) Front Immunol. 9:367
2. Clevers H, et al. (1988) Annu Rev Immunol. 6:629-62
3. Kuhns MS, Badgandi HB. (2012) Immunol Rev. 250(1):120-43
4. Wucherpfennig KW, et al. (2010) Cold Spring Harb Perspect Biol. 2(4):a005140
5. Samelson LE. (2002) Annu Rev Immunol. 20:371-94
6. Borroto A, et al. (2014) Immunol Lett. 161(1):113-7
Flow Cytometry
IHC
IHC
Immunoprecipitation Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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