Anti-Mouse TCR β chain – Purified in vivo GOLD™ Functional Grade

Clone H57-597 is a hamster monoclonal antibody targeting the beta chain of the mouse ?/? T cell receptor (TCR). In in vivo mouse studies, it is primarily used for selective depletion of ?/? T cells and modulation of T cell function.

Key applications and mechanisms in vivo include:

• T cell depletion: Systemic administration of H57-597 causes potent and rapid depletion of peripheral TCR ?/?+ T cells (including both CD4+ and CD8+ subsets), making it a standard tool for functional studies where researchers want to acutely remove these cells to study their roles in immune responses.

• Immune modulation and tolerance induction: Transient in vivo treatment with H57-597 not only reduces T cell numbers but also increases the frequency of regulatory T cells (Tregs) among remaining CD4+ cells and can induce transplant tolerance. For example, short-term H57-597 administration led to long-term cardiac allograft survival (>100 days) in allogeneic transplant models, thought to be mediated in part through Treg expansion and persistent unresponsiveness to donor antigens.

• Inhibition of antigen-specific responses: In models of superantigen challenge or transplantation, H57-597 selectively blocks the expansion of T cells expressing the targeted TCR V? chain while sparing non-targeted cells, allowing mechanistic dissection of TCR-specific immune responses.

• Phenotyping and cell tracking: H57-597 is also frequently used as a phenotypic marker to identify and track ?/? T cells in vivo, though flow cytometric or immunohistologic detection is more common ex vivo or in fixed tissue.

Special consideration: H57-597 is specific for TCR ?/? and does not react with TCR ?/? cells, allowing selective targeting of the main peripheral T cell pool without affecting ?/? T cells.

Summary table of major in vivo usages:

For in vivo use, low-endotoxin, carrier/protein-free preparations such as those provided by commercial vendors are often recommended to avoid confounding immune effects.

The correct storage temperature for sterile packaged clone H57-597 is between 2°C and 8°C, undiluted, and protected from light. Do not freeze this antibody solution.

Essential context:

• All major suppliers (BioLegend, OriGene) specify 2–8°C as the storage temperature for both purified and conjugated versions of H57-597.
• Some suppliers indicate that, for short term use, storage at 4°C is sufficient; for long-term storage, aliquoting and storing at -20°C is possible, but only if freeze/thaw cycles are strictly avoided. However, most guidance recommends not freezing and storing only at 2–8°C for routine lab use, especially for pre-formulated, ready-to-use sterile packs.
• Protect the antibody from prolonged light exposure and avoid multiple freeze/thaw cycles.

Summary of best practices:

• Store undiluted at 2°C–8°C
• Do not freeze
• Protect from light
• For long-term storage (>1 month), consult product-specific instructions and consider aliquoting at -20°C only if validated by supplier and if repeated thawing can be prevented

H57-597 is an Armenian hamster monoclonal antibody specific for the ?-chain of the mouse ?? T cell receptor (TCR?). In the literature, when using H57-597 to identify or isolate T cells, researchers commonly combine it with other antibodies or proteins to define T cell subsets, activation states, or co-receptor expression. Commonly used antibodies and proteins with H57-597 include:

• CD3: A core component of the TCR complex and a classic T cell marker. Anti-CD3 (e.g., clone 145-2C11) is often used in combination to define total T cells or for functional assays such as activation.
• CD4: Identifies helper T cells (Th cells). Staining for CD4 distinguishes CD4+ T cells within the TCR?+ population.
• CD8: Marks cytotoxic T lymphocytes (CTLs). Anti-CD8 antibodies are frequently used to separate CD8+ T cells among the TCR?+ compartment.
• CD44, CD62L: Memory and activation markers. Co-staining with these helps define naïve, effector, and memory T cell subsets within TCR?+ T cells.
• CD25, CD69, CD127: Activation and regulatory markers. These help identify activated or regulatory T cells when used with H57-597 staining.
• Viability dyes: Such as Zombie Aqua or 7-AAD, commonly included to exclude dead cells in flow cytometry protocols.
• Other lineage or exclusion markers: Such as B220 (B cells), NK1.1 (NK cells), Gr-1 (granulocytes), and CD11b (myeloid), allowing negative gating to define pure T cell populations.
• Tetramers or MHC multimers: Used to identify antigen-specific TCR?+ T cells, particularly in immunological studies.

These combinations enable detailed characterization of mouse T cell populations in both basic research and immunological studies, allowing discrimination by lineage (CD4 vs. CD8), activation state, memory differentiation, and antigen specificity.

Clone H57-597 is a monoclonal antibody specific for the beta chain of the mouse T-cell receptor (TCR ?/?), extensively cited in scientific literature for immunological research. Key findings from cited use of H57-597 are:

• T cell activation and depletion: H57-597 activates T cells when immobilized and can also be used for in vivo depletion of TCR ?/? positive T cells, supporting its utility in experiments requiring either stimulation or removal of T cells.

• Phenotypic marker: The antibody serves as a reliable marker for mouse T cells expressing the ?? TCR complex, enabling identification and quantification in flow cytometry and histological studies. It does not cross-react with ?? TCR-expressing cells.

• Functional applications: H57-597 is widely used to cross-link surface TCRs on T cells, mimicking antigen engagement and leading to cell activation, proliferation, differentiation, cytokine production, or activation-induced cell death. This makes it foundational in studies of TCR signaling pathways and T cell biology.

• Immunomodulation and transplantation tolerance: Transient administration of H57-597 monoclonal antibody in vivo induces profound immunological effects. Notably, in a heart transplant model, H57-597 markedly prolonged allograft survival (>100 days) compared to controls. This effect was associated with:

  • Initial depletion of conventional CD4? and CD8? T cells
  • Enrichment of CD4?FoxP3? regulatory T cells (Tregs)
  • Reduction of anti-donor T cell proliferative responses
  • Selective immune tolerance toward donor antigens while retaining broader immune responsiveness
  • The long-term tolerance was dependent on Treg cells; immune regulatory effects were reduced when Tregs were experimentally depleted.

• Autoimmunity models: Literature also reports H57-597 efficacy in suppressing experimental autoimmune encephalomyelitis and collagen-induced arthritis, attributed to its T cell depleting and immunoregulatory effects.

In summary, H57-597 is a critical reagent for mouse T cell detection, functional modulation, and immunological intervention, particularly valuable for studies of T cell depletion, activation, transplantation tolerance, and autoimmune regulation.