Anti-Mouse TIM-3 (Clone B8.2C12) – Purified in vivo GOLD™ Functional Grade

Anti-Mouse TIM-3 (Clone B8.2C12) – Purified in vivo GOLD™ Functional Grade

Product No.: T751

- -
- -
Clone
B8.2C12
Target
Tim-3
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Alternate Names
HAVCR2
Isotype
Rat IgG1 κ
Applications
FA
,
FC

- -
- -
Select Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Recommended Isotype Controls
Recommended Dilution Buffer
Immunogen
Mouse TIM-3 protein
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Liquid
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Additional Applications Reported In Literature ?
FA,
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
B8.2C12 activity is directed against mouse TIM-3 (CD366).
Background
TIM-3 is a member of the T cell immunoglobulin mucin gene family and encodes a type I membrane protein consisting of an immunoglobulin variable-region-like domain, a mucin-like domain, and a tyrosine phosphorylation motif1. TIM-3 functions as an important immune checkpoint receptor that helps regulate dendritic cell function2, T helper type I expansion, and induction of peripheral tolerance3. TIM-3 interacts with GAL-9, PtdSer, HMGB1 and CEACAM1 to activate biochemical pathways such as immune tolerance, T cell depletion, NF-κB signaling, and IL-2 secretion4. Additionally, TIM-3 expression correlates with terminal differentiation and exhaustion in tumors as well as chronic infection3. Due to its dysregulation in different types of cancer, TIM-3 blockade is being investigated as an anti-tumor immunotherapy2,4. TIM-3 also has potential as a prognostic marker in solid tumors4.

B8.2C12 was generated by immunizing Lewis and Lou/M female rats (Harlan Sprague-Dawley) with Th1 polarized T cell clones and/or lines, including Th1-specific clone AE7 and in vitro differentiated Th1 cell lines derived from 5B6 and DO11.10 T cell receptor transgenic mice1. Spleen cells were fused with myeloma cells and a large panel of monoclonal antibodies was screened on Th1 and Th2 cells by flow cytometry. B8.2C12 selectively stained Th1 cells. Gene expression cloning was then used to identify a complementary DNA, which was TIM-3.
Antigen Distribution
TIM-3 (CD366) is expressed on interferon-γ producing T cells, dendritic cells, cytotoxic lymphocytes, exhausted T cells, natural killer cells, Th17, and myeloid cells. TIM-3 is also expressed on CD8 + T cells in the tumor microenvironment as well as intratumoral macrophages and monocytes. TIM-3 is expressed by T helper 1 cells after several rounds of polarization in vitro. TIM-3 is not expressed by naïve T cells.
Ligand/Receptor
Expressed on activated Th1 lymphocytes, CD11b+ macrophages, and dendritic cells.
NCBI Gene Bank ID
UniProt.org
Research Area
Cell Biology
.
Immunology
.
Immune Checkpoint

References & Citations

1 Monney L, Sabatos CA, Gaglia JL, et al. Nature. 415(6871):536-541. 2002.
2 Dixon KO, Tabaka M, Schramm MA, et al. Nature. 595(7865):101-106. 2021.
3 Sabatos CA, Chakravarti S, Cha E, et al. Nat Immunol. 4(11):1102-1110. 2003.
4 Sauer N, Janicka N, Szlasa W, et al. Cancer Immunol Immunother. 72(11):3405-3425. 2023.
5 del Rio ML, Cote-Sierra J, Rodriguez-Barbosa JI. Transpl Int. 24(5):501-513. 2011.
6 Cong J, Wang X, Zheng X, et al. Cell Metab. 28(2):243-255.e5. 2018.
7 Chen L, Yang QC, Li YC, et al. Cancer Immunol Res. 8(2):179-191. 2020.
8 Taniguchi H, Caeser R, Chavan SS, et al. Cell Rep. 39(7):110814. 2022.
9 Guo J, De May H, Franco S, et al. Nat Biomed Eng. 6(1):19-31. 2022.
FA
Flow Cytometry

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.