Anti-Neurofilament-P – Purified

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Pricing & Details

Product No.N105
Clone
7H11/R3
Protein
Neurofilament-P
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse
IgM
Applications
IHC FFPE
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
N105-0.125 mg
0.125 mg
$254.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-Neurofilament (Mr 200 kDa-Phosphorylated) recognizes the phosphorylated form of the large subunit of the neurofilament triplet. The reactivity of this antibody is removed upon treatment of neurofilaments with alkaline phosphatase.
Antigen Distribution
The 200 kDa neurofilament-P antigen is present in neurons and tumor cells of neuronal origin.
Background
The family of proteins making intermediate filaments is divided into 5 major classes, the keratins forming the classes I and II. The neurofilament subunits occupy the class IV family of intermediate filaments, and was originally thought to contain only three proteins named NF-L, NF-M and NF-H. These names come from the apparent molecular weight of the mammalian subunits on SDS-PAGE:
- the light or lowest (NF-L) runs at 68-70kDa
- the medium or middle (NF-M) runs at about 145-160kDa
- the heavy or highest (NF-H) runs at 200-220kDa
The SDS-PAGE molecular weights vary between mammalian species, with larger species usually having larger proteins. Neurofilaments are found in vertebrate neurons in especially high concentrations along the axons, where they appear to regulate axonal diameter.

In the adult mammal neurofilament subunit proteins coassemble in vivo, forming a heteropolymer that contain NF-L plus NF-M or NF-H. The NF-H and NF-M proteins have lengthy C-terminal tail domains that appear to control the spacing between neighboring filaments, generating aligned arrays with a fairly uniform interfilament spacing.

Antigen Details

Research Area
Cell Biology
.
Neuroscience

References & Citations

Drager, U. C. et al. (1984) J. of Neuroscience 4:2025
IHC
General Western Blot Protocol

Formats Available

Prod No.
Description
N105
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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