This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Anti-Neurofilament (Mr 200 kDa-Phosphorylated) recognizes the phosphorylated form of the large subunit of the neurofilament triplet. The reactivity of this antibody is removed upon treatment of neurofilaments with alkaline phosphatase.
The 200 kDa neurofilament-P antigen is present in neurons and tumor cells of neuronal origin.
The family of proteins making intermediate filaments is divided into 5 major classes, the keratins forming the classes I and II. The neurofilament subunits occupy the class IV family of intermediate filaments, and was originally thought to contain only three proteins named NF-L, NF-M and NF-H. These names come from the apparent molecular weight of the mammalian subunits on SDS-PAGE: - the light or lowest (NF-L) runs at 68-70kDa - the medium or middle (NF-M) runs at about 145-160kDa - the heavy or highest (NF-H) runs at 200-220kDa The SDS-PAGE molecular weights vary between mammalian species, with larger species usually having larger proteins. Neurofilaments are found in vertebrate neurons in especially high concentrations along the axons, where they appear to regulate axonal diameter. In the adult mammal neurofilament subunit proteins coassemble in vivo, forming a heteropolymer that contain NF-L plus NF-M or NF-H. The NF-H and NF-M proteins have lengthy C-terminal tail domains that appear to control the spacing between neighboring filaments, generating aligned arrays with a fairly uniform interfilament spacing.
References & Citations
Drager, U. C. et al. (1984) J. of Neuroscience 4:2025
Products are for research use only. Not for use in diagnostic or therapeutic procedures.