Anti-Piccolo (PCLO) Antibody (Clone 6H9-B6)

Full-length human Piccolo protein (accession no. BC001304.1)

Phosphate-buffered saline (PBS), pH 7.4, containing 50% glycerol and 0.09% sodium azide.

Liquid

Purified by Protein G affinity chromatography

This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.

Research Use Only

Next Day 2-8°C

This antibody recognizes human and rat Piccolo isoforms (other species not yet tested). It does not cross-react with Bassoon.

Piccolo is a presynaptic cytomatrix protein localized at the presynaptic side of synaptic junctions. It is a large, multi-domain protein composed of an N-terminal zinc finger (Zn²⁺), several Piccolo-Bassoon homology (PBH) domains, and C-terminal PDZ and C2 domains. Piccolo is commonly found in association with Bassoon, a structurally related scaffolding protein, and both are key components of the cytomatrix at the active zone (CAZ).

As a scaffolding protein, Piccolo plays a critical role in organizing molecules involved in the endocytosis and exocytosis of synaptic vesicles. Recent studies have shown that Piccolo can modulate clathrin-mediated endocytosis by interacting with Abp1, an actin- and dynamin-binding protein.

Piccolo is highly expressed in the brain, with lower expression observed in the stomach. It is not detected in other tissues such as the adrenal gland, testis, or pancreas.

Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:19812333). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (By similarity). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Organizes as well the readily releasable pool of synaptic vesicles and safeguards a fraction of them to be not immediately available for action potential-induced release (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy (PubMed:28231469) (By similarity). Mediates also synapse to nucleus communication leading to rUniProtKB:Q9JKS6, PubMed:19812333, PubMed:28231469}.