Anti-Rat GFRα-1 (Clone 81401)
Pricing & Details
Product No.G674 Clone 81401 Protein GFR alpha -1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names GFRA1, GDNFR, GDNFRA, GFR-ALPHA-1, MGC23045, RET1L, RETL1, TRNR1, Glial Cell Line-Derived Neurotropic Factor soluble Receptor Alpha 1 Isotype Mouse IgG2b Applications IHC FFPE , WB  |
Antibody DetailsProduct DetailsReactivity Species Rat Host Species Mouse Immunogen NS0-Derived Recombinant Rat GFRα-1 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830263 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat GFRα-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat GFRα-1 is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Other Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Rat GDNF Family Receptor Alpha 1 (GFRα-1) (Clone 81401) recognizes an epitope on Rat GFRα-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rmRet, rhGFRα-2, rhGFRα-3 and rmGFRα-2. Background GDNF family receptor alpha 1, also known as GFRA1 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRalpha1 functions as a ligand-induced cell adhesion molecule (LICAM) to establish precise synaptic contacts and induce presynaptic differentiation.1 Antigen DetailsProtein PubMed References & Citations1. Ibáñez, CF. et al. (2007)Nat Neurosci. 10 : 293 Technical Protocols  |
Formats Available
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