Anti-Rat MAG (Clone 118423)

NS0-Derived Recombinant Rat MAG Extracellular Domain

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: This antibody is useful for neutralization of Rat MAG bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Rat MAG is 40 µg/ml.

Mouse Anti-Rat Myelin-Associated Glycoprotein (MAG) (Clone 118423) recognizes an epitope on Rat MAG. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Myelin-associated glycoprotein (MAG) is a transmembrane proteins that is a component of myelin in the central and peripheral nervous system.¹ It promotes neurite outgrowth during the embryonic development, but inhibits axonal regeneration in the adult nervous system.² MAG mediates cell-cell interactions between myelinating glial cells and neurons.³ MAG as an important component of the myelin-derived inhibition of nerve regeneration. The acute loss of MAG function can promote significant axon growth across a site of CNS nerve damage.⁴

1. Ming, GL. et al. (2008) Mol Brain. 1:10
2. Kelm, S. et al. (2001) FEBS Lett. 499: 262
3. Del Rio, JA. et al. (2005) Mol Cell Neurosci. 29: 471
4. Jay, DG. et al. (2003) J of Neurosci. 23: 3112