Anti-Rat MAG – Biotin
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Goat Immunogen Purified Recombinant Rat MAG (Accession # P07722) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831306 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat MAG this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Rat MAG is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M1243. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/mL. The recommended secondary antibody for IHC is PN:A106. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Rat Myelin-Associated Glycoprotein (MAG) recognizes Rat MAG. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Myelin-associated glycoprotein (MAG) is a transmembrane proteins that is a component of myelin in the central and peripheral nervous system.1 It promotes neurite outgrowth during the embryonic development, but inhibits axonal regeneration in the adult nervous system.2 MAG mediates cell-cell interactions between myelinating glial cells and neurons.3 MAG as an important component of the myelin-derived inhibition of nerve regeneration. The acute loss of MAG function can promote significant axon growth across a site of CNS nerve damage.4 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Ming, GL. et al. (2008) Molecular Brian 1: 10 2. Kelm, S. et al. (2001) FEBS Lett. 499: 262 3. Del Rio, JA et al. (2005) Mol Cell Neurosci. 29: 471 4. Jay, DG. et al. (2003) J of Neurosci. 23: 3112 Technical ProtocolsELISA Det IHC FFPE  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
