Anti-Rat MAG (Clone 118423)
Anti-Rat MAG (Clone 118423)
Product No.: M1279
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Clone 118423 Target MAG Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Siglec-4a, GMA Isotype IgG2a Applications N , WB |
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Mouse Immunogen NS0-Derived Recombinant Rat MAG Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831273 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat MAG this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat MAG is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Rat MAG bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat MAG is 40 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Rat Myelin-Associated Glycoprotein (MAG) (Clone 118423) recognizes an epitope on Rat MAG. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Myelin-associated glycoprotein (MAG) is a transmembrane proteins that is a component of myelin in the central and peripheral nervous system.1 It promotes neurite outgrowth during the embryonic development, but inhibits axonal regeneration in the adult nervous system.2 MAG mediates cell-cell interactions between myelinating glial cells and neurons.3 MAG as an important component of the myelin-derived inhibition of nerve regeneration. The acute loss of MAG function can promote significant axon growth across a site of CNS nerve damage.4 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Ming, GL. et al. (2008) Mol Brain. 1:10 2. Kelm, S. et al. (2001) FEBS Lett. 499: 262 3. Del Rio, JA. et al. (2005) Mol Cell Neurosci. 29: 471 4. Jay, DG. et al. (2003) J of Neurosci. 23: 3112 Technical ProtocolsN  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
