Anti-SARS-CoV-2 Spike

Pricing & Details

Product No.LT3500
Clone
2143
Protein
SARS-CoV-2 Spike
Formats AvailableView All
Product Type
Recombinant Monoclonal Antibody
Alternate Names
COV2-2143, SARS-CoV-2 Spike Antibody
Isotype
Human IgG1
Applications
ELISA
Prod No.
Size
Price
Avail.
Qty
Add to cart
LT3500-100 µg
100 µg
$395.00
In stock
Max:
Min: 1
Step: 1
LT3500-500 µg
500 µg
$795.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
SARS-CoV-2
Virus
Expression Host
HEK-293
Immunogen
Sequenced from human survivors of COVID-19 (SARS-CoV-2)
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Standard Overnight on Blue Ice.
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-SARS-CoV-2 Spike, clone 2143, specifically targets an epitope on the SARS-CoV-2 full length Spike protein (not specific to RBD or NTD).
Antigen Distribution
The spike is expressed on the surface of SARS-CoV-2.
Background
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), is an enveloped, single-stranded, positive-sense RNA virus that belongs to the Coronaviridae family 1. The SARS-CoV-2 genome, which shares 79.6% identity with SARS-CoV, encodes four essential structural proteins: the spike (S), envelope (E), membrane (M), and nucleocapsid protein (N) 2. The S protein is a transmembrane, homotrimeric, class I fusion glycoprotein that mediates viral attachment, fusion, and entry into host cells 3. Each ~180 kDa monomer contains two functional subunits, S1 (~700 a.a) and S2 (~600 a.a), that mediate viral attachment and membrane fusion, respectively. S1 contains two major domains, the N-terminal (NTD) and C-terminal domains (CTD). The CTD contains the receptor-binding domain (RBD), which binds to the angiotensin-converting enzyme 2 (ACE2) receptor on host cells 3-5.

Antigen Details

Research Area
COVID-19
.
Viral

References & Citations

1. Zhou, P., Yang, X., Wang, X. et al. Nature 579, 270–273. 2020.
2. Wu, F., Zhao, S., Yu, B. et al. Nature 579, 265–269. 2020.
3. Wrapp D, Wang N, Corbett KS, et al. bioRxiv. 2020.02.11.944462. 2020.
4. Walls AC, Park YJ, Tortorici MA, Wall A, McGuire AT, Veesler D. Cell. 181(2):281-292.e6. 2020.
5. Li W, Zhang C, Sui J, et al. EMBO J. 24(8):1634-1643. 2005.
Elisa Sandwich Protocol

Formats Available

Prod No.
Description
LT3500
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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