Anti-Shigella flexneri 2a, LPS (SHIG-8964)

Anti-Shigella flexneri 2a, LPS (SHIG-8964)

Product No.: S243

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Clone
SHIG-8964
Target
LPS
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Isotype
Mouse IgG1
Applications
ELISA
,
IF

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Select Product Size
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Antibody Details

Product Details

Reactivity Species
Shigella flexneri
Host Species
Mouse
Product Concentration
≥1.0 mg/ml
Purity
≥90%
Formulation
Formulated in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Liquid
Product Preparation
This monoclonal antibody is purified by protein A chromatography or sequential differential precipitations.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2-8°C Wet Ice
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA: 1:20-1:200,
IF: 1:10-1:50
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-Shigella flexneri (Clone SHIG-8964) is specific for the LPS of Shigella flexneri 2a and displays no cross reactivity for Shigella flexneri 1a, 3a, 4a, 5a, 6, S. sonnei, S. dysenteriae, S. boydii, Citrobacter, Salmonella (mix) or E. coli (mix).
Matched Pair
Best ELISA pair (capture/detection), SHIG-8964/SHIG-3914
Background
Shigella flexneri serotype 2a is a specific strain of Shigella flexneri responsible for causing shigellosis, a prominent global gastrointestinal infection. Serotype 2a holds clinical importance due to its prevalence and virulence. Shigella flexneri 2a infections often lead to severe symptoms such as bloody diarrhea, abdominal cramps, and fever1. The lipopolysaccharides (LPS) on the bacterial cell surface play a pivotal role in its pathogenicity by contributing to adhesion, immune evasion, and triggering inflammatory responses in the host. The bacterium's ability to invade the colonic epithelium leads to localized inflammation and tissue damage, causing the characteristic symptoms of shigellosis. Recently, there have been reports of increased transmission of extensively drug-resistant (XDR) Shigella spp. through sexual activity2.

Antigen Details

Research Area
Bacteria
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Category B Pathogens
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Infectious Disease
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Matched Pair

References & Citations

1. Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Division of Foodborne, Waterborne, and Environmental Diseases. Link
2. Caldera J, Yang S, Uslan DZ. Extensively Drug-Resistant Shigella flexneri 2a, California, USA, 2022. Emerging Infectious Diseases. 2023;29(7):1473-1475. doi:10.3201/eid2907.230465.
Indirect Elisa Protocol
IF

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.