Anti-Thymidylate Synthase [Clone 3A7.A11]

Anti-Thymidylate Synthase [Clone 3A7.A11]

Product No.: 34002


Clone 3A7.A11 Target Thymidylate synthase Formats AvailableView All Product Type Monoclonal Alternate Names TS, TSase, EC 2.1.1.45 Isotype Mouse IgG1 Applications ELISA , IHC , IP , WB


Select Product Size 200 µg — $447.00	Qty Max: Min: 1 Step: 1 $447.00 ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Recombinant human thymidylate synthase produced in E. coli. Product Concentration Lot Specific Formulation PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunohistochemistry: use at 5-10 ug/ml on frozen sections (reactivity on paraffin-embedded sections has not been determined) Immunoblotting: use at 5-10 ug/ml ELISA: use at 2-5 ug/ml (optimized for TS on solid phase at 5 ug/ml). Immunoprecipitation: use #34002 only at 5-10 ug/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Monoclonal Antibody specific to Human Thymidylate Synthase (36 kDa). It does not cross react with rat TS. Background Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time. Function Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway. {PubMed:11278511, PubMed:21876188}. NCBI Gene Bank ID TYMS UniProt.org P04818 Research Area Cancer Research References & Citations View product citations for antibody 34002 on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Recombinant human thymidylate synthase produced in E. coli.

Product Concentration

Lot Specific

Formulation

PBS, pH 7.4.

State of Matter

Liquid

Product Preparation

Purified by Protein G affinity chromatography

Storage and Handling

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

Immunohistochemistry: use at 5-10 ug/ml on frozen sections (reactivity on paraffin-embedded sections has not been determined)

Immunoblotting: use at 5-10 ug/ml

ELISA: use at 2-5 ug/ml (optimized for TS on solid phase at 5 ug/ml).

Immunoprecipitation: use #34002 only at 5-10 ug/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Monoclonal Antibody specific to Human Thymidylate Synthase (36 kDa). It does not cross react with rat TS.

Background

Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.

Function

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway. {PubMed:11278511, PubMed:21876188}.

NCBI Gene Bank ID

TYMS

UniProt.org

P04818

Research Area

Cancer Research