Anti-Ubiquitin Antibody
Data
Detection of ubiquitin in mouse brain lysate.
Immunohistochemistry: use at 1-10ug/ml. Detection of ubiquitin in human brain cortex, senile plaque. - -
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Antibody DetailsProduct DetailsReactive Species E. coli ⋅ Bovine ⋅ Canine ⋅ Chicken ⋅ Drosophila ⋅ Fish ⋅ Guinea Pig ⋅ Hamster ⋅ Human ⋅ Monkey ⋅ Mouse ⋅ Porcine ⋅ Rabbit ⋅ Rat ⋅ Sheep ⋅ Xenopus ⋅ Yeast Host Species Rabbit Immunogen Native bovine ubiquitin conjugated to KLH. Product Concentration Lot Specific Formulation PBS, pH 7.2, 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by protein A affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Store in appropriate aliquots to avoid repeated freeze-thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: Use at 1-5ug/mL. A band of ~10kDa is detected.
Immunohistochemistry: use at 1-10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes free ubiquitin as well as ubiquinated proteins in immunoblots. It recognizes human, monkey, mouse, rat, hamster, rabbit, Guinea pig, bovine, porcine, canine, sheep, chicken, Xenopus, yeast, Drosophila, and fish ubiquitin. Background Ubiquitin is a highly-conserved, 76-amino acid polypeptide expressed in all eukaryotic cells. Ubiquitination of proteins facilitates targeting of cytosolic and nuclear proteins for degradation by proteasomes. This essential cellular process involves a multi-enzyme cascade of ubiquitin- activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs), and ubiquitin-protein ligases (E3s). Ubiquitination is also involved in internalization and degradation of plasma membrane proteins such as T cell receptor subunits while still associated with membranes of the endoplasmic reticulum. NCBI Gene Bank ID Research Area Apoptosis References & Citations1. Li, B., Li, M., et al. Aging (Albany NY) . 2019 Dec 26 11(24):12546-12567 2. Garcia-Jove Navarro, M., Basset, C., et al. PLoS One . 2013 Aug 7 8(8):e71443 3. Ribeiro, J. R., Lovasco, L. A., et al. Front Oncol . 2014 Mar 11 4:45 4. Bouwman, R. D., Palser, A., et al.Retrovirology. 2014 Jul 3 11:53 5. Leonard, A., Paton, A. W., et al. PLoS One . 2014 Oct 30 9(10):e110949 6. Kang, K. A., Piao, M. J., et al. Oncotarget. 2016 Jun 28 7(26):40594-40620 7. Cai, T., Sun, D., et al. J Cell Mol Med. 2018 Mar 22(3):1684-1695 8. López de la Oliva, A. R., Campos-Sandoval, J. A., et al.Sci Rep. 2020 Feb 10 10(1):2259 9. Lei, W., Duron, D. I., et al.Front Mol Neurosci. 2019 Nov 29 12:294 Technical Protocols   Certificate of Analysis |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
