Anti-V5 Antibody
Data
Detection of V5-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-V5 antibody 18870 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. - -
- -
Antibody DetailsProduct DetailsHost Species Rabbit Immunogen Synthetic peptide corresponding to aa 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian virus 5 conjugated to KLH. Product Concentration 1.0 mg/ml Formulation Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide State of Matter Liquid Product Preparation Purified by peptide immuno-affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at 2-8°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at a dilution of 1:1,000- 1:20,000.
Immunocytochemistry: use at a dilution of 1:100-1:400. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500 These are recommended dilutions. Endusers should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to V5. Antibody was affinity-purified on the peptide. Background The V5 epitope tag represents aa 95-108 (GKPIPNPLLGLDST) derived from RNA polymerase alpha subunit of simian virus 5. Antigen DetailsResearch Area Epitope Tags References & CitationsTsurumi A et al. 2011. PLoS Genetics 7: e1002086 Xia F et al. 2008. BloS Biology 6: e128. Technical Protocols ICC   |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
