Anti-Viral CMV UL146 – Biotin

Anti-Viral CMV UL146 – Biotin

Product No.: C1515

[product_table name="All Top" skus="C1515"]

- -
- -
Target
CMV UL146
Product Type
Polyclonal Antibody
Alternate Names
Cytomegalovirus Unique Long 146 , Y18, vCXC1
Applications
WB

- -
- -
Select Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
viral
Host Species
Goat
Immunogen
E. coli-Derived Recombinant Viral CMV UL146
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Viral CMV UL146 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Viral CMV UL146 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Viral Cytomegalovirus UL 146 (CMV UL146) recognizes Viral CMV UL146. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Cytomegalovirus unique long 146 (CMV UL146) is a ubiquitous beta-herpes virus and is principally present in peripheral monocytes. It is the major infectious cause of birth defects.1 CMV has 19 genes designated as UL133 - UL151, of which UL146 is one of those genes that has lost virulence characteristics.

Antigen Details

PubMed

References & Citations

1. Stoncek, D. et al. (2004) Yonsei Med J. 45: 18
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.