Armenian Hamster IgG Isotype Control [Clone PIP] — Purified in vivo PLATINUM™ Functional Grade
Armenian Hamster IgG Isotype Control [Clone PIP] — Purified in vivo PLATINUM™ Functional Grade
Product No.: P376
Antibody DetailsProduct DetailsHost Species Armenian Hamster Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Working Concentration This isotype control antibody should be used at the same concentration as the primary antibody. RRIDAB_2894150 Applications and Recommended Usage? Quality Tested by Leinco FC This isotype control antibody should be used at the same concentration as the primary antibody. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Armenian Hamster IgG isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Furthermore, this Low Endotoxin Functional Formulation, PLATINUM antibody is suitable for in vivo application and each lot is IMPACT I certified. Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone PIP is commonly used as an Armenian Hamster IgG isotype control antibody in in vivo experiments in mice, as well as to study the roles of the prolactin-inducible protein (PIP) in various biological processes. Key in vivo applications of clone PIP in mice include:
Additional context:
In summary, clone PIP’s core in vivo applications in mice are as a highly validated IgG isotype control and as an investigative tool for understanding the role of prolactin-inducible protein in immune responses, cancer, and host defense. Commonly used antibodies or proteins with PIP in the literature depend heavily on the context—whether PIP refers to phosphatidylinositol phosphate(s) (usually phosphoinositides such as PI(4)P, PI(4,5)P2, etc.) or to prolactin-induced protein (also known as BRST-2, Gp17, or simply PIP). Both major usages have distinct sets of associated antibodies and proteins. 1. Phosphatidylinositol Phosphate (PIP) and Related Antibodies/ProteinsWhen PIP refers to phosphoinositides:
2. Prolactin-Induced Protein (PIP) and Related Antibodies/ProteinsWhen PIP refers to prolactin-induced protein, especially in cancer or breast biology:
3. Other Relevant Antibody Combinations and Protein Partners
Summary Table — Common Associated Antibodies/Proteins (by PIP context):
The most relevant antibodies or proteins used alongside PIP are selected based on biological pathway or experimental context, with those above representing the major groupings in published research. The term "PIP" appears across diverse areas of scientific research, each with distinct key findings depending on the specific context. Here are the major discoveries from different PIP-related studies: Plant Peptide ResearchIn plant biology, PIP (Plant Immunogenic Peptide) family research has revealed significant functional divergence between subfamilies. AtPIP1 demonstrates the strongest root growth inhibition activity, while AtPIP2 shows superior pathogen resistance capabilities. This functional distinction is partially attributed to the SGP motif, where proline hydroxylation modification enhances PIP function. The research suggests that AtPIP2 could potentially be used for crop protection in agricultural settings, as it induces immunity more efficiently without causing severe root growth penalties. Breast Cancer BiologyStudies on Prolactin-Induced Protein (PIP) in breast cancer have uncovered its crucial role in regulating tumor cell proliferation. When PIP was silenced in luminal A breast cancer cells, researchers observed decreased phosphorylation of several key proteins including focal adhesion kinase (FAK), ephrin B3 (EphB3), FYN, and hemopoietic cell kinase. The PIP-activated gene network forms intricate connections with cMYC and cJUN, which serve as master transcriptional regulators of cell proliferation. Additionally, high PIP expression levels have been shown to sensitize breast cancer cells to anti-cancer drugs, enhancing treatment efficacy. Microbial PathogenesisIn bacterial research, the pip gene was identified as essential for producing the antifungal metabolite phenazine-1-carboxamide (PCN) in Pseudomonas chlororaphis. Mutants lacking functional pip failed to produce this important antimicrobial compound. Pathotype IdentificationThe PIP-eco pipeline represents a genomic tool for E. coli pathotype identification, achieving a 70.9% assignment rate for single pathotypes and successfully identifying hybrid pathotypes in bacterial populations. This tool has proven particularly effective in detecting ExPEC/AIEC groups and their hybrid combinations with other pathotypes. Currently, there is limited specific information available on how dosing regimens of clone PIP vary across different mouse models. However, general principles in dosing regimens for mouse models can be inferred from similar studies:
Without direct information on "Clone PIP," it is challenging to provide specific dosing regimens for this clone across different mouse models. It is essential to consult specific research articles or protocols related to "Clone PIP" for detailed dosing information. References & Citations1. Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117. PubMed 2. Oldstone, MBA. et al. (2017) Proc Natl Acad Sci U S A. 114(14): 3708–3713. PubMed 2. Schreiber, RD. et al. (2015) PLoS One.10(5):e0128636. PubMed Technical ProtocolsCertificate of Analysis |
Related Products
Prod No. | Description |
---|---|
C2841 |
Formats Available
Prod No. | Description |
---|---|
I-905 | |
I-445 | |
I-446 | |
I-897 | |
I-898 | |
I-899 | |
I-901 | |
I-902 | |
I-903 | |
I-448 | |
I-447 | |
I-140 | |
P376 | |
I-140-FAB2 |
