Name: BriClone SF (Serum Free) Hybridoma Cloning Medium 20x
SKU: BR-002
Price: 450.00 USD
Availability: InStock

BriClone SF (Serum Free) Hybridoma Cloning Medium 20x

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BriClone SF (Serum Free) Hybridoma Cloning Medium 20x

Product No.: BR-002


Applications Cell Culture


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Data

Percentage of fusion efficiency from freshly fused hybridomas cultured using BriClone (red) compared to Bri-Clone-SF (brown) and a negative control (blue).

BriClone-SF is stable for at least 9 months when stored at -20˚C. Percentage of fusion efficiency from freshly fused hybridomas cultured using BriClone-SF stored at 4˚C for 2 weeks, Room Temperature for 1 week and -20˚C for 9 months compared to a negative control (blue).

BriClone-SF has equivalent fusion efficiency activity when compared to two leading competitor products when used at the manufacturer’s recommended concentrations. Percentage of fusion efficiency from freshly fused hybridomas cultured using BriClone-SF (orange) compared to Competitor A (light grey) and competitor B (dark grey) products and a negative control (blue).

Antibody Details

Product Details

Product Concentration

20X Solution

Formulation

Dulbeccos Modified Eagle medium (DMEM): Non Animal Source

Foetal Bovine Serum: origin United States of America, certified free of viruses

State of Matter

Liquid

Storage and Handling

Store at -20°C. Avoid repeated freeze/thaw cycles. Stable for short periods at +4°C

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

Thaw and add to the hybridoma cloning medium as a 5% v/v supplement. BriClone SF can be used for:
• Hybridoma growth post-fusion (refer to your own protocol)
• Hybridoma Cloning (refer to your own protocol)

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

BriClone SF Hybridoma Cloning Medium

Background

BriClone is produced by exposing basal culture medium with a low percentage of fetal calf serum to a human cancer cell line, which secretes relevant molecules into the medium, resulting in a product consistently supportive of the fusion and cloning stages of hybridoma production. Some researchers require a product which is serum-free and has had no contact with human or animal cells. We have, therefore, designed BriClone-SF which has the same basal medium and biological activities as BriClone but has no added serum or other substances of human or animal origin and has not been exposed to cells. BriClone-SF contains added recombinant human proteins produced in bacterial or yeast host cells.

References & Citations

PMC7873262,PMC7239657,PMC6803335,PMC6816435,PMC6719973,PMC5017023,PMC2812581,PMC2896538,PMC4617144,PMC3944398,PMC9030397,PMC8470961,PMC8104110,PMC7279471,PMC7096428,PMC5992998,PMC5986985,PMC5857169,PMC4921032,PMC4591809,PMC4135088,PMC1858089,PMC1602225

View product citations for antibody BR-002 on CiteAb

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