ClearSight Sheath Fluid

ClearSight is a premium, 1X concentrated isotonic buffered sheath fluid optimized for high-performance flow cytometry applications. To ensure optimal baseline and protect fluidic lines, the formulation is calcium-free, magnesium-free, and protein-free. The manufacturing process incorporates a rigorous mixing sequence followed by a final fill 0.22 µm PES membrane filtration step to eliminate particulate matter.

Instrument Compatibility and Performance
The fluid is engineered for seamless integration with acoustic focusing systems and has been comprehensively ran on platforms such as the Attune™ NxT Flow Cytometer. Comparative evaluation against standard focusing fluids demonstrates full functional equivalence across critical cytometric parameters. Validation testing highlights include:

- System Readiness: Instruments successfully complete standard startup and performance verification cycles immediately upon onboarding the fluid.

- Hydrodynamic Stability: The fluid maintains consistent operational performance across varied sample throughput speeds, including low (100 µl/min), medium (500 µl/min), and high (1000 µl/min) flow rates.

Signal Replication: Comparative overlay analysis shows identical forward scatter (FSC) and side scatter (SSC) profiles. Additionally, the fluid yields perfectly aligned fluorescence peaks across blue, violet, yellow, and red laser parameters, ensuring data integrity and preventing streaming-related artifacts.

Manufactured in St. Louis, MO, USA under strict quality control guidelines to ensure excellent lot-to-lot consistency and performance reproducibility. The manufacturing process utilizes standard operating procedures featuring a comprehensive automated mixing sequence followed by a validated 0.22 µm PES membrane filtration step to guarantee a low particulate solution. Each production batch is strictly verified against rigid specifications for appearance, pH, and functional density to maintain maximum performance stability across all compatible systems.

7.30–7.40.

1X Liquid "Ready Use"

This product consists of an aqueous, isotonic buffered matrix designed to maintain baseline stream and fluidic stability in Flow Cytometry cell analysis. To preserve chemical and functional integrity, the solution incorporates a non-sodium azide preservative system, offering a safer alternative for laboratory handling and disposal compared to traditional azide-containing reagents. The formulation is strictly free of calcium, magnesium, and cellular proteins.