Purified Hamster IgG (H+L) (>98%)
This secondary antibody is formulated in sterile 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl.
Storage and Handling
This antibody is stable for at least one year when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
One year from date of receipt
Applications and Recommended Usage ?
(Quality Tested by Leinco)
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
IHC (NBF/Par.): This antibody may be used to stain nuclear membranes in cells, tissue preparations or subcellular fractions. The antibody works well with cells and tissue sections fixed with 2% paraformaldehyde and requires no dilution. Tonsil is the recommended positive control tissue.
Flow Cytometry: PN:A106
Western Blotting: To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:M114 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.
Goat Whole Molecule Anti-Syrian Hamster IgG (H&L) recognizes Hamster IgG. This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.