Purified Human IgG (H+L) (>98%)
This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase.)
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Storage and Handling
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. Caution: This horseradish peroxidase conjugate does not contain preservatives. To avoid microbial contamination, store the reconstituted conjugate containing glycerol at -20°C in a manual defrost freezer.
One year from date of reconstitution
Country of Origin
Next Day Ambient
It is recommended that working dilutions be prepared fresh daily. WB: 1:5,000 - 1:100,000WB Chemiluminescent: 1:10,000 - 1:200,000ELISA: 1:5,000 - 1:100,000IHC: 1:500 - 1:5,000Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat F(ab')2 Anti-Human IgG (H+L) recognizes Human IgG (H+L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.