Molecular Biology Grade Water

Molecular Biology Grade Water

Product No.: W2020

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Applications
Cell Culture
,
Immunoassays
,
PCR

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Select Product Size
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Product Details

pH Range
5.0 - 7.0
Storage and Handling
Store at room temperature.

Description

Background
Our Molecular Biology Grade Water is a high-purity reagent engineered for sensitive genomic and proteomic research. Using a rigorous multi-stage purification process—including Reverse Osmosis (RO), Deionization, and UV-C Sterilization—this water is refined to meet the most exacting standards. Each lot is processed through 0.1 μm filtration and specialized endotoxin-removal filters to ensure a sterile, nuclease-free environment for your most critical samples.

Features and Benefits
- Multi-Stage Purification: Utilizes RO and DI technology for consistent ionic purity.

- Endotoxin-Free: Specifically filtered to remove pyrogens, making it ideal for cell-related molecular work.

- Nuclease & Protease-Free: Guaranteed absence of DNase, RNase, and Protease to protect nucleic acid and protein integrity

- UV-C Treated: Continuous ultraviolet irradiation ensures the highest level of microbial control.

- Non-DEPC Treated: Clean, chemical-free processing that prevents interference with enzymatic reactions or downstream sequencing.

Benefits
1. Ultra-High Purity & Versatility
Our water is refined through a rigorous multi-step process that eliminates ionic and organic contaminants. This high-purity profile makes it a "universal" reagent, ensuring that your results are never skewed by trace minerals or chemical artifacts.

Consistency: Every lot is standardized to ensure reproducible results across different experiments.

Safety: 100% DEPC-free, avoiding the potential for chemical modification of your samples or interference with enzymatic activity.

2. Maximum Biological Integrity (Nuclease & Protease-Free)
The presence of even trace amounts of enzymes can ruin weeks of work. Leinco water is strictly validated to be:
- RNase & DNase-Free: Critical for the preservation of delicate RNA and DNA templates.

- Protease-Free: Essential for protein purification, Western Blotting, and any application involving sensitive enzymes or antibodies.

3. Low Endotoxin Levels for Sensitive Cell Work
Unlike standard molecular water, our product undergoes specific Endotoxin removal and 0.1 µm filtration.

- Cell Culture Ready: Low endotoxin levels ensure that the water is non-toxic to sensitive cell lines, making it ideal for media preparation and transfection protocols.

- Pyrogen-Free: Meets the strict requirements for applications where cellular responses must be monitored without background interference from bacterial byproducts.
Specifications
Test Specification Result
Appearance (Color & Odor) Colorless and Odorless Observed result
Appearance (Turbidity) Clear Observed result
Dnases None Detected Test result reported
Rnases None Detected Test result reported
Nickase None Detected Test result reported
pH 5.0 to 7.0 Test result reported
Conductivity < 1.3 µS/cm at 25°C Observed value reported
Endotoxins ≤ 1 EU/mL Test result reported
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Hazard Description
GHS Classification: Not a hazardous substance or mixture.

Skin & Eye Contact:While non-irritating, use of standard personal protective equipment (PPE) such as gloves and safety glasses is recommended to prevent accidental contamination of the product.

Other Safety and Handling: NON-POTABLE.This product is intended for laboratory research use only. It is produced in a facility that is not registered for food-grade production. It is not for human or animal consumption, nor is it intended for medicinal or diagnostic use.

Manufacturing Standards

The quality and reliability of Leinco’s Molecular Biology Grade Water are anchored in our state-of-the-art manufacturing facility located in St. Louis, Missouri. This facility operates under a rigorous Quality Management System that is certified to ISO 13485:2016 standards, ensuring that every milliliter of water produced meets the highest global benchmarks for medical device and life science reagent manufacturing. Our commitment to excellence is reflected in our move away from traditional distillation toward a sophisticated, multi-stage purification architecture. By integrating Reverse Osmosis (RO), high-capacity Deionization (DI), and continuous UV-C germicidal irradiation, we achieve a level of ionic and organic purity that exceeds industry norms.

The St. Louis facility is designed with high-efficiency particulate air (HEPA) filtered cleanrooms where all bottling and aseptic filling occur. This controlled environment is critical to maintaining the biological inertness of our product, specifically preventing the introduction of ambient nucleases or proteases that could compromise sensitive downstream applications. To further guarantee the safety and stability of the reagent, the water is processed through specialized endotoxin-removal filters and a final 0.1 μm absolute filtration step. This specialized process ensures the water is not only sterile but also pyrogen-free, making it ideal for everything from delicate cell culture to complex genomic sequencing.

Leinco’s commitment to quality extends beyond the manufacturing line to a comprehensive suite of analytical testing. Every lot undergoes stringent validation for the absence of DNase, RNase, and Protease activity, as well as bioburden and endotoxin analysis. Because our process is entirely chemical-free and does not utilize diethyl pyrocarbonate (DEPC), researchers can trust that there are no residual contaminants to interfere with enzymatic reactions. This dedication to purity, backed by our ISO-certified infrastructure in St. Louis, ensures that Leinco provides a foundational reagent characterized by absolute consistency and traceability for the global scientific community.

Related Protocols

Cell Culture
Immunoassays
PCR
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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.